Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 52519

HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85 NL-43)
Epitope	SLYNTVATL	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	class I down-regulation by Nef, escape

Notes

• NL43 was passaged in the presence of Nef KEKGGLEGL-specific CTL clones STD11 and KM3, giving rise to rapid selection of escape mutations, including E93G, E93K, K94N+G99R, G95R+G99R, E98K, E98D, G99R, G99E, L100P, and L100I; insertions, deletions, frameshifts and an early stop codon. 34/36 (94%) of sequences carried mutations in the epitope by seven days, 36/36 (100%) by 14 days.
• Several mutations selected by KEKGGLEGL-specific CTL were shown to impair the down regulation of class I MHC by Nef, in particular E93G, E93K, and a truncation mutation at position 51.
• Nef deletion mutants increased 100-fold NL-43 susceptibility to inhibition by CTL specific for epitopes in other proteins, the A2 epitopes ILKEPVHGV in RT and SLYNTVATL in p17 Gag.

References

Ali2003 Ayub Ali, Satish Pillai, Hwee Ng, Rachel Lubong, Douglas D. Richman, Beth D. Jamieson, Yan Ding, M. Juliana McElrath, John C. Guatelli, and Otto O. Yang. Broadly Increased Sensitivity to Cytotoxic T Lymphocytes Resulting from Nef Epitope Escape Mutations. J. Immunol., 171(8):3999-4005, Oct 2003. PubMed ID: 14530319. Show all entries for this paper.

Displaying record number 52518

HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	RT(309-317 NL-43)
Epitope	ILKEPVHGV	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	class I down-regulation by Nef, escape

Notes

• NL43 was passaged in the presence of Nef KEKGGLEGL-specific CTL clones STD11 and KM3, giving rise to rapid selection of escape mutations, including E93G, E93K, K94N+G99R, G95R+G99R, E98K, E98D, G99R, G99E, L100P, and L100I; insertions, deletions, frameshifts and an early stop codon. 34/36 (94%) of sequences carried mutations in the epitope by seven days, 36/36 (100%) by 14 days.
• Several mutations selected by KEKGGLEGL-specific CTL were shown to impair the down regulation of class I MHC by Nef, in particular E93G, E93K, and a truncation mutation at position 51.
• Nef deletion mutants increased 100-fold NL-43 susceptibility to inhibition by CTL specific for epitopes in other proteins, the A2 epitopes ILKEPVHGV in RT and SLYNTVATL in p17 Gag.

References

Ali2003 Ayub Ali, Satish Pillai, Hwee Ng, Rachel Lubong, Douglas D. Richman, Beth D. Jamieson, Yan Ding, M. Juliana McElrath, John C. Guatelli, and Otto O. Yang. Broadly Increased Sensitivity to Cytotoxic T Lymphocytes Resulting from Nef Epitope Escape Mutations. J. Immunol., 171(8):3999-4005, Oct 2003. PubMed ID: 14530319. Show all entries for this paper.

Displaying record number 52516

HXB2 Location	Nef(92-100) DNA(9070..9096)	Nef Epitope Map
Author Location	Nef(92-100 NL-43)
Epitope	KEKGGLEGL	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B60)
Immunogen	HIV-1 infection
Experimental methods
Keywords	class I down-regulation by Nef, escape

Notes

• NL43 was passaged in the presence of Nef KEKGGLEGL-specific CTL clones STD11 and KM3, giving rise to rapid selection of escape mutations, including E93G, E93K, K94N+G99R, G95R+G99R, E98K, E98D, G99R, G99E, L100P, and L100I; insertions, deletions, frameshifts and an early stop codon. 34/36 (94%) of sequences carried mutations in the epitope by seven days, 36/36 (100%) by 14 days.
• NL43 was also passaged in the presence of a Nef TQGYFPDWQNY-specific CTL clone. 7/15 clones had a frameshifting or stop codon introduced by one week; F121T was also observed. The most common escape mutation for both Nef epitopes was an early stop codon at position 91.
• Several mutations selected by KEKGGLEGL-specific CTL were shown to impair the down regulation of class I MHC by Nef, in particular E93G, E93K, and a truncation mutation at position 51.
• Nef deletion mutants increased 100-fold NL-43 susceptibility to inhibition by CTL specific for epitopes in other proteins, the A2 epitopes ILKEPVHGV in RT and SLYNTVATL in p17 Gag.

References

Ali2003 Ayub Ali, Satish Pillai, Hwee Ng, Rachel Lubong, Douglas D. Richman, Beth D. Jamieson, Yan Ding, M. Juliana McElrath, John C. Guatelli, and Otto O. Yang. Broadly Increased Sensitivity to Cytotoxic T Lymphocytes Resulting from Nef Epitope Escape Mutations. J. Immunol., 171(8):3999-4005, Oct 2003. PubMed ID: 14530319. Show all entries for this paper.

Displaying record number 52520

HXB2 Location	Nef(117-127) DNA(9145..9177)	Nef Epitope Map
Author Location	Nef(117-127 NL-43)
Epitope	TQGYFPDWQNY	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*1501)
Immunogen	HIV-1 infection
Experimental methods
Keywords	class I down-regulation by Nef, escape

Notes

• NL43 was passaged in the presence of Nef KEKGGLEGL-specific CTL clones STD11 and KM3, giving rise to rapid selection of escape mutations, including E93G, E93K, K94N+G99R, G95R+G99R, E98K, E98D, G99R, G99E, L100P, and L100I; insertions, deletions, frameshifts and an early stop codon. 34/36 (94%) of sequences carried mutations in the epitope by seven days, 36/36 (100%) by 14 days.
• NL43 was also passaged in the presence of a Nef TQGYFPDWQNY-specific CTL clone. 7/15 clones had a frameshifting or stop codon introduced by one week; F121T was also observed. The most common escape mutation for both Nef epitopes was an early stop codon at position 91.
• Several mutations selected by KEKGGLEGL-specific CTL were shown to impair the down regulation of class I MHC by Nef, in particular E93G, E93K, and a truncation mutation at position 51.
• Nef deletion mutants increased 100-fold NL-43 susceptibility to inhibition by CTL specific for epitopes in other proteins, the A2 epitopes ILKEPVHGV in RT and SLYNTVATL in p17 Gag.

References

Ali2003 Ayub Ali, Satish Pillai, Hwee Ng, Rachel Lubong, Douglas D. Richman, Beth D. Jamieson, Yan Ding, M. Juliana McElrath, John C. Guatelli, and Otto O. Yang. Broadly Increased Sensitivity to Cytotoxic T Lymphocytes Resulting from Nef Epitope Escape Mutations. J. Immunol., 171(8):3999-4005, Oct 2003. PubMed ID: 14530319. Show all entries for this paper.