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Displaying record number 4143

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MAb ID	1E5
HXB2 Location	gp160	gp160 Epitope Map
Author Location	gp120
Epitope
Subtype	CRF02_AG
Ab Type	gp120 C1-C2
Neutralizing	no
Species (Isotype)	human(IgG1, IgG3)
Patient	KMCB2
Immunogen	HIV-1 infection
Country	Japan
Keywords	ADCC, antibody binding site, antibody generation, antibody interactions, antibody sequence, binding affinity

Notes

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1E5: MAb 1E5 was isolated from a patient infected with CRF02_AG, and both the IGg1 and IGg3 forms were produced. Its binding region was determined to be the C1-C2 region of gp120. In a binding competition assay, 1E5 enhanced rather than competed for A32 binding, suggesting that the 1E5 epitope does not overlap with the A32 epitope. Neither the IgG1 nor the IgG3 forms of 1E5 showed any neutralization activity, similar to the other C1C2 antibodies. Both IgG1 and IgG3 forms of 1E5 showed low ADCC activity against most of the strains, although 1E5-IgG3 showed higher ADCC activity than 1E5-IgG1. 1E5 ADCC activity was enhanced in the presence of A32, 4E9C, and anti-CoRBS antibodies. The 1E5 antibody sequence and germline gene usage were determined. MdZahid2021 (ADCC, antibody binding site, antibody generation, antibody interactions, binding affinity, antibody sequence)

References

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Isolation Paper
MdZahid2021 Hasan Md Zahid, Takeo Kuwata, Shokichi Takahama, Yu Kaku, Shashwata Biswas, Kaho Matsumoto, Hirokazu Tamamura, and Shuzo Matsushita. Functional analysis of a monoclonal antibody reactive against the C1C2 of Env obtained from a patient infected with HIV-1 CRF02_AG. Retrovirology, 18(1):23 doi, Aug 2021. PubMed ID: 34419098 Show all entries for this paper.