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Displaying record number 4016

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MAb ID	E10
HXB2 Location	gp160(653-663) DNA(8181..8213)	gp160 Epitope Map
Author Location	gp41
Epitope	QEKNEQELLEL	Epitope Alignment
Ab Type	gp41 MPER (membrane proximal external region)
Neutralizing	L
Species (Isotype)	human(IgG1)
Patient
Immunogen	HIV-1 infection
Country	China
Keywords	ADCC, antibody generation, neutralization

Notes

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• E10: This study has isolated the human mAb E10 from an HIV-1-infected patient sample by single B cell sorting and single cell PCR. E10 bound to the gp140 trimer and linear peptides derived from gp41 membrane proximal external region (MPER). E10 binds to peptide F7 (amino acid: QQELLELDKWANLWNWFDISNWLW, 657–680, HXB2 numbering). This epitope (QEKNEQELLEL) overlapped with mAb 2F5 epitope. However, E10 however was different from 2F5 in neutralization breadth and potency, as well as ADCC activity. E10 showed low neutralization activity and a narrow spectrum of neutralization compared to 2F5, but it mediated higher ADCC activity than 2F5 at low antibody concentration. Fine mapping of the E10 epitope may potentiate MPER-based subunit vaccine development. Yang2018 (ADCC, antibody generation, neutralization)

References

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Isolation Paper
Yang2018 Zheng Yang, Xi Liu, Zehua Sun, Jingjing Li, Weiguo Tan, Weiye Yu, and Meiyun Zhang. Identification of a HIV gp41-Specific Human Monoclonal Antibody with Potent Antibody-Dependent Cellular Cytotoxicity. Front. Immunol., 9:2613, 2018. PubMed ID: 30519238. Show all entries for this paper.