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Displaying record number 3486

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MAb ID	JR4
HXB2 Location	Env	Env Epitope Map
Author Location	Env( SHIV KB9)
Epitope
Ab Type	gp120 C1-C2
Neutralizing
Species (Isotype)	macaque
Patient
Immunogen	SHIV infection
Keywords	ADCC, antibody binding site, structure

Notes

Showing 2 of 2 notes.

JR4: The study isolated the inner domain (ID) of gp120 as an independent molecule that encapsulates the epitope region recognized by A32-like MAbs. The construct ID2 consists of the ID stabilized in the CD4-bound conformation. The crystal structure of A32 Fab in complex with ID2 revealed the epitope of A32. The study also determined the structure of ID2 in complex with JR4, a C1-C2-specific MAb. Tolbert2016 (structure)
JR4: Antibody JR4 was derived from PBMC of a rhesus macaque infected with SHIV KB9 with deletions of glycosylation sites in gp41. Initial epitope assignment was based on competition experiments with MAbs A32 and C11. JR4 was strongly positive for ADCC activity. The structure of JR4 in complex with gp120 was determined. Gohain2015 (ADCC, antibody binding site, structure)

References

Showing 2 of 2 references.

Gohain2015 Neelakshi Gohain, William D. Tolbert, Priyamvada Acharya, Lei Yu, Tongyun Liu, Pingsen Zhao, Chiara Orlandi, Maria L. Visciano, Roberta Kamin-Lewis, Mohammad M. Sajadi, Loïc Martin, James E. Robinson, Peter D. Kwong, Anthony L. DeVico, Krishanu Ray, George K. Lewis, and Marzena Pazgier. Cocrystal Structures of Antibody N60-i3 and Antibody JR4 in Complex with gp120 Define More Cluster A Epitopes Involved in Effective Antibody-Dependent Effector Function against HIV-1. J. Virol., 89(17):8840-8854, Sep 2015. PubMed ID: 26085162. Show all entries for this paper.

Tolbert2016 William D. Tolbert, Neelakshi Gohain, Maxime Veillette, Jean-Philippe Chapleau, Chiara Orlandi, Maria L. Visciano, Maryam Ebadi, Anthony L. DeVico, Timothy R. Fouts, Andres Finzi, George K. Lewis, and Marzena Pazgier. Paring Down HIV Env: Design and Crystal Structure of a Stabilized Inner Domain of HIV-1 gp120 Displaying a Major ADCC Target of the A32 Region. Structure, 24(5):697-709, 3 May 2016. PubMed ID: 27041594. Show all entries for this paper.