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Displaying record number 1006

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MAb ID	AG11
HXB2 Location	Nef(194-206) DNA(9376..9414)	Nef Epitope Map
Author Location	Nef( LAI)
Research Contact	Frank Jirik, Centre for Molecular Med and Therapeutics, U. B. C., Vancouver, B. C. Canada
Epitope	VARELHPEYFKNC	Epitope Alignment
Subtype	B
Ab Type	C-term
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

Showing 1 of 1 note.

• AG11: The light and heavy chains of three MAbs (AG11, AE6, EH1) specific to C-terminus of NEF were cloned and variable regions sequenced -- the complementarity determining regions (CDR) of AG11 and AE6 were highly related (95.1% at the DNA level) and bound LAI Nef, but not SF2 Nef -- EH1 bound to SF2 and LAI and cross-competed AG11 and AE6 but had a distinctive CDR (57.9% similar to AG11) -- single chain Abs were constructed from AG11 and EH1 and subcloned into a eukaryotic expression vector with a green fluorescent protein marker to allow intracellular expression -- the single chain Abs bind Nef intracellularly and may be useful to better understand the role of Nef and as a gene therapy model. Chang1998

References

Showing 1 of 1 reference.

Chang1998 A. H. Chang, J. A. Hoxie, S. Cassol, M. O'Shaughnessy, and F. Jirik. Construction of Single-Chain Antibodies That Bind an Overlapping Epitope of HIV-1 Nef. FEBS Lett., 441:307-312, 1998. PubMed ID: 9883905. Show all entries for this paper.

Displaying record number 1007

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MAb ID	AE6
HXB2 Location	Nef(194-206) DNA(9376..9414)	Nef Epitope Map
Author Location	Nef( LAI)
Research Contact	Frank Jirik, Centre for Molecular Med and Therapeutics, U. B. C., Vancouver, B. C. Canada
Epitope	VARELHPEYFKNC	Epitope Alignment
Subtype	B
Ab Type	C-term
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

Showing 3 of 3 notes.

• AE6 database note: NIH AIDS Research and Reference Reagent Program: 709 and 1123
• AE6: Similarity level of the AE6 binding site pentapeptide HPEYF to the host proteome was low, with the low-similarity 5-mer occurring in the host proteome 0 times, indicating that this peptide can be used to elicit Abs for active/passive immunotherapy with low risk of cross-reaction with the host proteome. Kanduc2008
• AE6: The light and heavy chains of three MAbs (AG11, AE6, EH1) specific to C-terminus of NEF were cloned and variable regions sequenced -- the complementarity determining regions (CDR) of AG11 and AE6 were highly related (95.1% at the DNA level) and bound LAI Nef, but not SF2 Nef -- EH1 bound to SF2 and LAI and cross-competed AG11 and AE6 but had a distinctive CDR (57.9% similar to AG11) -- single chain Abs were constructed from AG11 and EH1. Chang1998

References

Showing 4 of 4 references.

Chang1998 A. H. Chang, J. A. Hoxie, S. Cassol, M. O'Shaughnessy, and F. Jirik. Construction of Single-Chain Antibodies That Bind an Overlapping Epitope of HIV-1 Nef. FEBS Lett., 441:307-312, 1998. PubMed ID: 9883905. Show all entries for this paper.

Kanduc2008 Darja Kanduc, Rosario Serpico, Alberta Lucchese, and Yehuda Shoenfeld. Correlating Low-Similarity Peptide Sequences and HIV B-Cell Epitopes. Autoimmun. Rev., 7(4):291-296, Feb 2008. PubMed ID: 18295732. Show all entries for this paper.

Greenway1994 A. L. Greenway, D. A. McPhee, E. Grgacic, D. Hewish, A. Lucantoni, I. Macreadie, and A. Azad. Nef 27, but not the Nef 25 Isoform of Human Immunodeficiency Virus-Type 1 pNL4.3 Down-Regulates Surface CD4 And IL-2R Expression in Peripheral Blood Mononuclear Cells and Transformed T Cells. Virology, 198:245-256, 1994. PubMed ID: 7903128. Show all entries for this paper.

Tornatore1994 C. Tornatore, K. Meyers, W. Atwood, K. Conant, and E. Major. Temporal patterns of human immunodeficiency virus type 1 transcripts in human fetal astrocytes. J. Virol., 68:93-102, 1994. PubMed ID: 8254781. Show all entries for this paper.