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Displaying record number 1827

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MAb ID	P1H6
HXB2 Location	gp160(143-148) DNA(6651..6668)	gp160 Epitope Map
Author Location	gp120( SF162)
Research Contact	Nina Derby or Leo Stamatatos, Seattle Biomedical Research Institute, Seattle, WA, USA, leo.stamatotos@sbri.org
Epitope	`SSNWKE`	Epitope Alignment
Subtype	B
Ab Type	gp120 V1
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Country	United States
Keywords	antibody binding site, neutralization

Vaccine Details

Vaccine type	DNA prime with protein boost
Vaccine strain	B clade SF162
Vaccine component	gp140ΔV2

Notes

Showing 2 of 2 notes.

P1H6: The study explores how the V1 loop of Env influences the neutralization susceptibilities of heterologous viruses to antibodies elicited by the SF162gp140 immunogen. When the V1 loop of the heterologous isolates was replaced by the V1 loop present on the DF162go140 immunogen, these isolates became susceptible to neutralization by p1H6. Ching2008 (neutralization)
P1H6: Binding of P1H6 is partially dependent on the conformation of V1, while the presence of V3 is not required. P1H6 neutralized SF162 potently but it did not have any heterologous neutralizing activity. The Ab did not neutralize virus lacking V1. The SF162ΔV2 virus was significantly more susceptible to neutralization by P1H6 than the wildtype virus. Glycans at positions 154 and 195 in V1V2 were involved in regulating P1H6 neutralizing potential. Neutralization by P1H6 was also enhanced strongly by deletion of the V3 glycan at position 299, even more so by deletion at position 329, and only slightly or not at all by deletion of the glycan at position 293. Glycans present in the V4-V5 region had only modest effects on the neutralizing potential of this Ab, where their removal resulted in a more neutralization resistant virus. Derby2007 (antibody binding site, neutralization)

References

Showing 2 of 2 references.

Isolation Paper
Derby2007 Nina R. Derby, Sean Gray, Elizabeth Wayner, Dwayne Campogan, Giorgos Vlahogiannis, Zane Kraft, Susan W. Barnett, Indresh K. Srivastava, and Leonidas Stamatatos. Isolation and Characterization of Monoclonal Antibodies Elicited by Trimeric HIV-1 Env gp140 Protein Immunogens. Virology, 366(2):433-445, 30 Sep 2007. PubMed ID: 17560621. Show all entries for this paper.

Ching2008 Lance K. Ching, Giorgos Vlachogiannis, Katherine A. Bosch, and Leonidas Stamatatos. The First Hypervariable Region of the gp120 Env Glycoprotein Defines the Neutralizing Susceptibility of Heterologous Human Immunodeficiency Virus Type 1 Isolates to Neutralizing Antibodies Elicited by the SF162gp140 Immunogen. J. Virol., 82(2):949-956, Jan 2008. PubMed ID: 18003732. Show all entries for this paper.