Search Antibody Database

Found 1 matching record:

Displaying record number 430

Download this epitope record as JSON.

MAb ID	5023A (5023, NEA-9205, NEA 9205)
HXB2 Location	gp160(311-317) DNA(7155..7175)	gp160 Epitope Map
Author Location	gp120(311-317 BH10)
Research Contact	Paul Durda, Du Pont de Nemours and Co
Epitope	`RGPGRAF`	Epitope Alignment
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing	L
Species (Isotype)	mouse(IgG)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation, binding affinity

Vaccine Details

Vaccine type	peptide
Vaccine strain	B clade BH10
Vaccine component	Env V3

Notes

Showing 5 of 5 notes.

5023A: Called NEA-9205 -- The N306 glycan of the V3 loop makes the tip of the V3 loop inaccessible to this MAb in oligomeric Env, loss of this glycan enhances neutralization sensitivity. Schonning1998
5023A: Called 5023 in this paper -- Used to precipitate gp160 in immunoblots in a study examining the feasibility of using unprocessed gp160 glycoprotein as an immunogen. Rovinski1995
5023A: Called 5023 -- Langedijk also has an MAb called 5023B -- gp41 amino acid substitutions 668 (N/S) and 675 (I/M) in gp41 interfere with 5023s neutralization potency, region 662-675 is ELDKWANLWNWFNI. Back1993
5023A: Called 5023 -- Langedijk also has an MAb called 5023B -- strong cross-reactive neutralizing MAb. DSouza1991
5023A: Generation and Fine mapping of murine MAbs. 5023A was raised in mice against PND (principal neutralization domain) RIQRGPGRAFV, specifically peptide RIQRGPGRAFVTIGK. Reactive epitope was RGPGRAFVTIG (311-321) with the core being the tripeptide RAF. Reactivity with the tetrapeptide GRAF was 3x that of RAF. There was no contribution to reactivity by 312G, 318V, 320I but all other residues had major contributions. Langedijk1991 (antibody binding site, antibody generation, binding affinity)

References

Showing 5 of 5 references.

Back1993 N. K. T. Back, L. Smit, M. Schutten, P. L. Nara, M. Tersmette, and J. Goudsmit. Mutations in Human Immunodeficiency Virus Type 1 gp41 Affect Sensitivity to Neutralization by gp120 Antibodies. J. Virol., 67:6897-6902, 1993. Three closely related clones were derived from a neutralization resistant IIIB isolate that had been passaged in a chimpanzee. gp41 mutations were shown to profoundly alter the ability of V3 loop MAbs 5023 and 178.1 to neutralize. Critical substitutions in gp41 were 668 and 675, close to the immunogenic domain 662-668, or ELDKWAS. Less profound inhibition was observed for the anti-CD4 binding site MAb GP13. PubMed ID: 8411395. Show all entries for this paper.

DSouza1991 M. P. D'Souza, P. Durda, C. V. Hanson, G. Milman, and Collaborating Investigators. Evaluation of Monoclonal Antibodies to HIV-1 by Neutralization and Serological Assays: An International Collaboration. AIDS, 5:1061-1070, 1991. PubMed ID: 1718320. Show all entries for this paper.

Langedijk1991 J. P. M. Langedijk, N. K. T. Back, P. J. Durda, J. Goudsmit, and R. H. Meloen. Neutralizing activity of anti-peptide antibodies against the principal neutralization domain of human immunodeficiency virus type 1. J. Gen. Virol., 72:2519-2526, 1991. MAbs were raised against RIQRGPGRAFVTIGK by immunizing mice. Fine structure of MAb binding sites was mapped using pepscan. Preservation of the $\beta$ turn at the tip of the loop was critical. PubMed ID: 1919529. Show all entries for this paper.

Rovinski1995 B. Rovinski, L. Rodrigues, S. X. Cao, F. L. Yao, U. McGuinness, C. Sia, G. Cates, S. Zolla-Pazner, S. Karwowska, T. J. Matthews, C. B. McDanal, J. Mascola, and M. H. Klein. Induction of HIV Type 1 Neutralizing and Env-CD4 Blocking Antibodies by Immunization with Genetically Engineered HIV Type 1-Like Particles Containing Unprocessed gp160 Glycoproteins. AIDS Res. Hum. Retroviruses, 11:1187-1195, 1995. PubMed ID: 8573374. Show all entries for this paper.

Schonning1998 K. Schonning, A. Bolmstedt, J. Novotny, O. S. Lund, S. Olofsson, and J. E. Hansen. Induction of Antibodies against Epitopes Inaccessible on the HIV Type 1 Envelope Oligomer by Immunization with Recombinant Monomeric Glycoprotein 120. AIDS Res. Hum. Retroviruses, 14:1451-1456, 1998. PubMed ID: 9824323. Show all entries for this paper.