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Displaying record number 823

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MAb ID	C8
HXB2 Location	gp160(727-732) DNA(8403..8420)	gp160 Epitope Map
Author Location	gp41(727-732 BH10)
Epitope	`PDRPEG`	Epitope Alignment
Subtype	B
Ab Type	C-term
Neutralizing	P (tier 1)
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	review

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade LAI
Vaccine component	gp160

Notes

Showing 7 of 7 notes.

C8 database comment: there are two distinct antibodies in the database named C8.
C8: Unlike SAR1, a MAb that binds near the C8 epitope within the Kennedy peptide, C8 cannot inhibit fusion between HIV-1 infected and target cells. C8 recognizes PDRPEG on the surface of HIV-1 infected cells, but not on virions and is non-neutralizing. Heap2005a
C8: This review summarizes the complex antigenic properties of an external loop in the gp41 tail (spanning the Kennedy sequence), highlighting specific MAbs. C8 binds to the epitope PDRPEG and does not neutralize virus. Dimmock2005 (review)
C8: The substitution 725 RG (P[R-->G]GPDRPEGIEEEGGERDRDRS) alters the antigenic exposure of this region on the virion resulting in the loss of the downstream neutralizing epitope ERDRD, increased exposure of the epitope GPDRPEG in the virion, while the epitope IEEE remains unchanged. McLain2001
C8: Epitope boundaries mapped by peptide scanning. Abacioglu1994
C8: Ab response in IIIB lab workers was compared to gp160 LAI vaccine recipients -- C8 was used as a control -- the dominant response among vaccinees was to this mid-gp41 region, but not among the infected lab workers -- Abs binding this region do not neutralize, bind to infected cells, nor serve as immunotoxins. Pincus1993a
C8: Immunotoxin of C8 coupled to ricin-A does not mediate cells killing, and is not affected by sCD4. Pincus1993

References

Showing 6 of 6 references.

Abacioglu1994 Y. H. Abacioglu, T. R. Fouts, J. D. Laman, E. Claassen, S. H. Pincus, J. P. Moore, C. A. Roby, R. Kamin-Lewis, and G. K. Lewis. Epitope Mapping and Topology of Baculovirus-Expressed HIV-1 gp160 Determined with a Panel of Murine Monoclonal Antibodies. AIDS Res. Hum. Retroviruses, 10:371-381, 1994. Thirty MAbs were obtained from BALB/c mice immunized with rgp160 LAI expressed in baculovirus. These antibodies map to 4 domains: gp120 C1, C2, C3/V4, and the cytoplasmic tail of gp41. All epitopes were exposed on rgp160 without denaturing the protein, but 6/8 epitopes mapped in gp120 are not exposed unless the protein is denatured, showing rgp160 and rgp120 fold differently. PubMed ID: 8068416. Show all entries for this paper.

Dimmock2005 Nigel J. Dimmock. The Complex Antigenicity of a Small External Region of the C-Terminal Tail of the HIV-1 gp41 Envelope Protein: A Lesson in Epitope Analysis. Rev. Med. Virol., 15(6):365-381, Nov-Dec 2005. PubMed ID: 16106492. Show all entries for this paper.

Heap2005a Caroline J. Heap, Steven A. Reading, and Nigel J. Dimmock. An Antibody Specific for the C-Terminal Tail of the gp41 Transmembrane Protein of Human Immunodeficiency Virus Type 1 Mediates Post-Attachment Neutralization, Probably Through Inhibition of Virus-Cell Fusion. J. Gen. Virol., 86(5):1499-1507, May 2005. PubMed ID: 15831963. Show all entries for this paper.

McLain2001 L. McLain, J. L. Brown, L. Cheung, S. A. Reading, C. Parry, T. D. Jones, S. M. Cleveland, and N. J. Dimmock. Different effects of a single amino acid substitution on three adjacent epitopes in the gp41 C-terminal tail of a neutralizing antibody escape mutant of human immunodeficiency virus type 1. Arch. Virol., 146(1):157--66, 2001. PubMed ID: 11266210. Show all entries for this paper.

Pincus1993 S. H. Pincus and J. McClure. Soluble CD4 Enhances the Efficacy of Immunotoxins Directed against gp41 of the Human Immunodeficiency Virus. Proc. Natl. Acad. Sci. U.S.A., 90:332-336, 1993. PubMed ID: 8419938. Show all entries for this paper.

Pincus1993a S. H. Pincus, K. G. Messer, D. H. Schwartz, G. K. Lewis, B. S. Graham, W. A. Blattner, and G. Fisher. Differences in the Antibody Response to Human Immunodeficiency Virus-1 Envelope Glycoprotein (gp160) in Infected Laboratory Workers and Vaccinees. J. Clin. Invest., 91:1987-1996, 1993. PubMed ID: 7683694. Show all entries for this paper.