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MAb ID	polyclonal
HXB2 Location	gp160(305-322) DNA(7137..7190)	gp160 Epitope Map
Author Location	gp140( SF162)
Epitope	KSITIGPGRAFYATGD	Epitope Alignment
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing
Species (Isotype)	macaque, rabbit(IgG)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

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• SF162ΔV2 is a virus that has a 30 amino acids deletion in the V2 loop that does not abrogate its infectivity but renders it highly susceptible to neutralization -- when incorporated into a codon-optimized DNA vaccine with a CMV promoter, delivered by gene gun, SF162Δ2 gave higher neutralizing Ab titers against SF162 than did SF162 itself, and Abs that cross-neutralized non-homologous primary isolates were obtained only when SF162ΔV2, but not intactSF162, was used as the immunogen -- NAbs titers specific for SF162 increased with multiple immunizations, while titers for non-homologous isolates decreased, but anti-V3 peptide binding Abs were not likely the source of this distinction because anti-V3 titers were much lower than those against the entire envelope, and the second booster immunization did not increase the titer of anti-V3 loop Abs. Barnett2001a

References

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Barnett2001a S. W. Barnett, S. Lu, I. Srivastava, S. Cherpelis, A. Gettie, J. Blanchard, S. Wang, I. Mboudjeka, L. Leung, Y. Lian, A. Fong, C. Buckner, A. Ly, S. Hilt, J. Ulmer, C. T. Wild, J. R. Mascola, and L. Stamatatos. The ability of an oligomeric human immunodeficiency virus type 1 (HIV-1) envelope antigen to elicit neutralizing antibodies against primary HIV-1 isolates is improved following partial deletion of the second hypervariable region. J. Virol., 75(12):5526--40, Jun 2001. URL: http://jvi.asm.org/cgi/content/full/75/12/5526. PubMed ID: 11356960. Show all entries for this paper.