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MAb ID	13-102-100
HXB2 Location	Gag(216-226) DNA(1435..1467)	Gag Epitope Map
Author Location	p24(102-112 IIIB)
Research Contact	Advanced Technologies, Inc., Columbia, MD
Epitope	HPVHAGPIAPG	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG)
Patient
Immunogen
Keywords

Notes

Showing 2 of 2 notes.

• 13-102-100: Affinity capillary electrophoresis was used to fine map this epitope, and the optimal peptide was defined as VHAGPIAPGIAP -- this method uses migration time shifts to probe relative affinities of Abs -- the antibody binds to the cyclophilin A binding domain. Qian1998
• 13-102-100: Binding site (HPVHAGPIAPG) defined by epitope footprinting -- first binding p24 to MAb, then allowing proteolytic cleavage to take place to cleave unprotected residues, then performing mass spectrometry to identify protected residues of epitope. Parker1996

References

Showing 2 of 2 references.

Parker1996 C. E. Parker, D. I. Papac, S. K. Trojak, and K. B. Tomer. Epitope Mapping by Mass Spectrometry: Determination of an Epitope on HIV-1 IIIB p26 Recognized by a Monoclonal Antibody. J. Immunol., 157:198-206, 1996. PubMed ID: 8683115. Show all entries for this paper.

Qian1998 X. H. Qian and K. B. Tomer. Affinity capillary electrophoresis investigation of an epitope on human immunodeficiency virus recognized by a monoclonal antibody. Electrophoresis, 19:415-9, 1998. PubMed ID: 9551794. Show all entries for this paper.