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Found 2 matching records:

Displaying record number 251

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MAb ID	1G7
HXB2 Location	Rev(96-105) DNA(8588..8617)	Rev Epitope Map
Author Location	Rev(95-105)
Epitope	GVGSPQILVE	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2bκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

Showing 2 of 2 notes.

• 1G7: Peptide interaction mapped to aa 91-105, 96-110 -- protein footprint to aa 95-105. Jensen1997
• 1G7: Worked in indirect immunofluorescence and also detected Rev in WB -- used to detect localization of Rev throughout the cell. Kalland1994

References

Showing 2 of 2 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

Displaying record number 250

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MAb ID	1G10 (IG10F4)
HXB2 Location	Rev(96-105) DNA(8588..8617)	Rev Epitope Map
Author Location	Rev(95-105)
Research Contact	Anne Marie Szilvay
Epitope	GVGSPQILVE	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2bκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

Showing 4 of 4 notes.

• 1G10: Similarity level of the 1G10 binding site pentapeptide SPQIL to the host proteome was low, with the low-similarity 5-mer occurring in the host proteome 1 time, indicating that this peptide can be used to elicit Abs for active/passive immunotherapy with low risk of cross-reaction with the host proteome. Kanduc2008
• 1G10: Called IG10F4: UK Medical Research Council AIDS reagent: ARP3060.
• 1G10: Peptide interaction mapped to aa 91-105, 96-110 -- protein footprint to aa 10-20, and 95-105. Jensen1997
• 1G10: Bound Rev in indirect immunofluorescence and also detected Rev in WB -- used to detect localization of Rev throughout the cell. Kalland1994

References

Showing 3 of 3 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

Kanduc2008 Darja Kanduc, Rosario Serpico, Alberta Lucchese, and Yehuda Shoenfeld. Correlating Low-Similarity Peptide Sequences and HIV B-Cell Epitopes. Autoimmun. Rev., 7(4):291-296, Feb 2008. PubMed ID: 18295732. Show all entries for this paper.