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Displaying record number 208

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MAb ID	4-20
HXB2 Location	Pol(968-976) DNA(4986..5012)	Pol Epitope Map
Author Location	Integrase(253-261 HXB2)
Research Contact	Yoshihiro Kitamura, Div of Mol Genetics, Nat Inst of Infectious Diseases, Musashimurayama, Japan
Epitope	DNSDIKVVP	Epitope Alignment
Subtype	B
Ab Type	Integrase DNA binding domain
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody polyreactivity, binding affinity, neutralization

Vaccine Details

Notes

Showing 3 of 3 notes.

• 4-20: Database comment: There are two different mAbs in the database with ID 4-20.
• 4-20: 134 unique anti-gp140 and 52 control non-gp140 reactive antibodies were studied for polyreactivity and its effect on binding affinity and neutralization. 75% of antibodies were polyreactive. 4-20 is not sensitive to neutralization and binds to dsDNA, LPS (lipopolysaccharide), insulin and CL (cardiolipin) and does not bind to ssDNA and ANA (antinuclear antibody). Mouquet2010 (neutralization, binding affinity, antibody polyreactivity)
• 4-20: Inhibits the terminal cleavage and strand transfer functions of Integrase, but not the disintegration activity. Ishikawa1999

References

Showing 2 of 2 references.

Ishikawa1999 T. Ishikawa, N. Okui, N. Kobayashi, R. Sakuma, T. Kitamura, and Y. Kitamura. Monoclonal Antibodies against The Minimal DNA-Binding domain in the Carboxy-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase. J. Virol., 73:4475-4480, 1999. PubMed ID: 10196350. Show all entries for this paper.

Mouquet2010 Hugo Mouquet, Johannes F. Scheid, Markus J. Zoller, Michelle Krogsgaard, Rene G. Ott, Shetha Shukair, Maxim N. Artyomov, John Pietzsch, Mark Connors, Florencia Pereyra, Bruce D. Walker, David D. Ho, Patrick C. Wilson, Michael S. Seaman, Herman N. Eisen, Arup K. Chakraborty, Thomas J. Hope, Jeffrey V. Ravetch, Hedda Wardemann, and Michel C. Nussenzweig. Polyreactivity Increases the Apparent Affinity of Anti-HIV Antibodies by Heteroligation. Nature, 467(7315):591-595, 30 Sep 2010. PubMed ID: 20882016. Show all entries for this paper.