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Displaying record number 218

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MAb ID	17 (mAb17)
HXB2 Location	Pol(740-750) DNA(4302..4334)	Pol Epitope Map
Author Location	Integrase(25-35)
Epitope	`DFNLPPVVAKE`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	review

Vaccine Details

Vaccine type	protein
Vaccine component	Int

Notes

Showing 4 of 4 notes.

17: Antibody inhibitors against HIV-1 integrase and the potential for antibody-based therapeutics are reviewed. Marchand2009 (review)
17: Epitope mapped to helix-turn-helix motif in the N-term domain of Integrase, positions 25-35 -- Zn binding stabilizes the Integrase-mAb17 complex -- both MAb and Fab form of mAb17 inhibit Integrase activity -- epitope region likely to be involved in protein-protein interaction. Yi2000b
17: Used for the creation of single chain variable antibody fragments (SFvs) for internal cellular expression -- neutralization of IN activity prior to integration, whether the Ab is expressed in the nucleolus or the cytoplasm -- relative binding affinity to IN: 12 > 17 = 33 > 21 > 4. Levy-Mintz1996
17: BALBc mice were immunized with rec integrase, hybridomas expressing anti-integrase Abs were generated, and the antibodies characterized -- the Zn finger motif is in the binding region -- MAbs 14 and 17 form a competition group. Bizub-Bender1994

References

Showing 4 of 4 references.

Bizub-Bender1994 D. Bizub-Bender, J. Kulkosky, and A. M. Skalka. Monoclonal Antibodies against HIV Type 1 Integrase: Clues to Molecular Structure. AIDS Res. Hum. Retroviruses, 10:1105-1115, 1994. PubMed ID: 7530024. Show all entries for this paper.

Levy-Mintz1996 P. Levy-Mintz, L. Duan, H. Zhang, B. Hu, G. Dornadula, M. Zhu, J. Kulkosky, D. Bizub-Bender, A. M. Skalka, and R. J. Pomerantz. Intracellular Expression of Single-Chain Variable Fragments to Inhibit Early Stages of the Viral Life Cycle by Targeting Human Immunodeficiency Virus Type 1 Integrase. J. Virol., 70:8821-8832, 1996. Comments in J Virol 1998 Apr;72(4):3505-6. PubMed ID: 8971011. Show all entries for this paper.

Yi2000b J izu Yi, Jonathan W. Arthur, Roland L. Dunbrack, Jr., and Anna Marie Skalka. An Inhibitory Monoclonal Antibody Binds at the Turn of the Helix-Turn-Helix Motif in the N-Terminal Domain of HIV-1 Integrase. J. Biol. Chem., 275(49):38739-38748, 8 Dec 2000. PubMed ID: 10969077. Show all entries for this paper.

Marchand2009 Christophe Marchand, Kasthuraiah Maddali, Mathieu Metifiot, and Yves Pommier. HIV-1 IN Inhibitors: 2010 Update and Perspectives. Curr. Top. Med. Chem., 9(11):1016-1037, 2009. PubMed ID: 19747122. Show all entries for this paper.