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Displaying record number 1878

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MAb ID	1G12
HXB2 Location	gp160(533-552) DNA(7821..7880)	gp160 Epitope Map
Author Location	gp41
Research Contact	Dr. Dennis Burton and Carlos Barbas
Epitope	`ASVTLTVQARLLLSGIVQQQ`	Epitope Alignment
Subtype	C
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody interactions, neutralization, variant cross-reactivity

Vaccine Details

Vaccine type	protein
Vaccine strain	C clade 97CN54
Vaccine component	other

Notes

Showing 2 of 2 notes.

1G12: The antibody was mapped to gp41/aa531-550. The capacity of 1G12 to block completely the activity of the anti-HIV peptide T20 was investigated. T20 inhibited the fusion or syncytia formation between co-cultured CHO-WT cells expressing HIV-1 HXB2 envelope glycoprotein on their surface and HeLaT4 cells. 1G12 blocked the anti-fusion effect of T20. Vincent2012 (antibody interactions)
1G12: Mice were immunized with a construct of the outer domain (OD) of gp120 of subtype C fused with Fc, and MAbs specific for the CN54 OD were derived by exhaustive screening of the mice sera. The OD-specific 1G12 MAb cross-competed with three other newly identified OD-specific MAbs: 4E1, 3F9, 1H8, but did not cross-compete with 4D3 (bridging sheet) or the V3-specific 2B7 and 4E5. 1G12 showed no neutralization of the three isolates tested, CN54, MN, and 93MW965.26. Chen2008a (neutralization, variant cross-reactivity)

References

Showing 2 of 2 references.

Chen2008a Hongying Chen, Xiaodong Xu, Hsin-Hui Lin, Ssu-Hsien Chen, Anna Forsman, Marlen Aasa-Chapman, and Ian M. Jones. Mapping the Immune Response to the Outer Domain of a Human Immunodeficiency Virus-1 Clade C gp120. J. Gen. Virol., 89(10):2597-2604, Oct 2008. PubMed ID: 18796729. Show all entries for this paper.

Vincent2012 Nadine Vincent and Etienne Malvoisin. Ability of Antibodies Specific to the HIV-1 Envelope Glycoprotein to Block the Fusion Inhibitor T20 in a Cell-Cell Fusion Assay. Immunobiology, 217(10):943-950, Oct 2012. PubMed ID: 22387075. Show all entries for this paper.