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Displaying record number 2459
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MAb ID |
HGN194 |
HXB2 Location |
Env(306-317) DNA(7140..7175) |
Env Epitope Map
|
Author Location |
gp120 |
Epitope |
RRSVRIGPGQTF
|
Epitope Alignment
|
Subtype |
AG |
Ab Type |
gp120 V3 // V3 glycan (V3g) |
Neutralizing |
P View neutralization details |
Contacts and Features |
View contacts and features |
Species
(Isotype)
|
human(IgG1) |
Patient |
VI3265 |
Immunogen |
HIV-1 infection |
Keywords |
adjuvant comparison, antibody binding site, antibody generation, assay or method development, binding affinity, escape, immunoprophylaxis, immunotherapy, mimotopes, neutralization, review, structure, subtype comparisons, variant cross-reactivity |
Notes
Showing 8 of
8 notes.
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HGN194: Env trimer BG505 SOSIP.664 as well as the clade B trimer B41 SOSIP.664 were stabilized using a bifunctional aldehyde (glutaraldehye, GLA) or a heterobifunctional cross-linker, EDC/NHS with modest effects on antigenicity and barely any on biochemistry or structural morphology. ELISA, DSC and SPR were used to test recognition of the trimers by bNAbs, which was preserved and by weakly NAbs or non-NAbs, which was reduced. Cross-linking partially preserves quaternary morphology so that affinity chromatography by positive selection using quaternary epitope-specific bNAabs, and negative selection using non-NAbs, enriched antigenic characteristics of the trimers. Binding of anti-V3 non-NAb HGN194 to trimers was reduced by trimer cross-linking.
Schiffner2016
(assay or method development, binding affinity, structure)
-
HGN194: Different adjuvants, including Freund's adjuvant (FCA/FIA), MF59, Carbopol-971P and 974P were compared on their ability to elicit antibody responses in rabbits. Combination of Carbopol-971P and MF59 induced potent adjuvant activity with significantly higher titer nAbs than FCA/FIA. There was no difference in binding of this MAb to gp140 SF162 with any of the adjuvants, as compared to the unadjuvanted sample.
Lai2012
(adjuvant comparison)
-
HGN194: mAbs with predetermined specificity were isolated from rhesus monkeys (RM) using differential biopanning method. Fluorescent mimotopes resembling V3 loop were used as baits to isolate single memory B cells. mAbs 33B2 and 33C6 were the best binders and neutralizers among 11 mABs. HGN194 was used as a positive control to compare 33B2 and 33C6 activities.
Sholukh2012
(mimotopes, neutralization, binding affinity)
-
HGN194: The potential of HGN194 to protect infant rhesus monkeys (RM) against mucosal challenge with a heterologous SHIV encoding a CCR5-tropic (R5) HIV-C envelope was evaluated. At a high nmAb HGN194 dose, all animals were completely protected, indicating for the first time potent cross-clade protection by a human anti-HIV-1 MAb in vivo. Additionally, all SHIV-challenged RM treated with high-dose HGN194 developed Gag-specific T-cell immunity.
Watkins2011a
(immunoprophylaxis, immunotherapy)
-
HGN194: Characteristics of patients' plasma and their respective mAbs in multiple neutralization assay formats was presented. HGN194 MAb neutralized SF162 (B), 92Br025 (C), VI 191 (A) and 89.6 (B) isolates against a panel of 17 primary viruses belonging to 6 subtypes in the 24/1/14 extended incubation PBMC assay. In another experiment, out of the 12 isolates, HGN194 MAb neutralized three in 24/1/14 PBMC assays, three in 1/2/7 PBMC assays, two in 1/24/14 PBMC assays, none in TZMbl_IV assays, four in TZMbl_PV assays and six in GHOST_PV assays. When evaluating plasma vs. Ab in the TZMbl pseudovirus assay, HGN194 MAb neutralized all three Tier 1 strains but neutralized only three out of eleven Tier 2 strains.
Balla-Jhagjhoorsingh2011
(neutralization, subtype comparisons)
-
HGN194: This review discusses current understanding of Env neutralization by antibodies in relation to epitope exposure and how this insight might benefit vaccine design strategies. This MAb is in the list of current MAbs with notable cross-neutralizing activity.
Pantophlet2010
(neutralization, variant cross-reactivity, review)
-
HGN194: This review outlines the general structure of the gp160 viral envelope, the dynamics of viral entry, the evolution of humoral response, the mechanisms of viral escape and the characterization of broadly neutralizing Abs. It is noted that this MAb, directed to the V3 loop, is mostly effective against neutralization sensitive viruses.
Gonzalez2010
(neutralization, variant cross-reactivity, escape, review)
-
HGN194: This MAb was isolated from memory B cells of HIV-1 infected donor using an improved EBV immortalization method combined with a broad screening strategy. HGN194 bound to Env proteins from clades A, AE, AG, B, BC, C, D and F. The epitope of this Ab was located in an extremely conserved region of the V3 crown and only three amino acid replacements in the epitope affected Ab binding. HGN194 neutralized with high potency all Tier 1 viruses of clades A, B, C, BC and AG, and 11% of Tier 2 viruses. In the HOS-based assay it neutralized 11/20 pseudoviruses and in the TZM-bl assay it neutralized 19/92 pseudoviruses. The neutralization potency of this Ab was greater than that of 447-52D.
Corti2010
(antibody binding site, antibody generation, neutralization, variant cross-reactivity, binding affinity, subtype comparisons)
References
Showing 8 of
8 references.
Isolation Paper
Corti2010
Davide Corti, Johannes P. M. Langedijk, Andreas Hinz, Michael S. Seaman, Fabrizia Vanzetta, Blanca M. Fernandez-Rodriguez, Chiara Silacci, Debora Pinna, David Jarrossay, Sunita Balla-Jhagjhoorsingh, Betty Willems, Maria J. Zekveld, Hanna Dreja, Eithne O'Sullivan, Corinna Pade, Chloe Orkin, Simon A. Jeffs, David C. Montefiori, David Davis, Winfried Weissenhorn, Áine McKnight, Jonathan L. Heeney, Federica Sallusto, Quentin J. Sattentau, Robin A. Weiss, and Antonio Lanzavecchia. Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals. PLoS One, 5(1):e8805, 2010. PubMed ID: 20098712.
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Balla-Jhagjhoorsingh2011
Sunita S. Balla-Jhagjhoorsingh, Betty Willems, Liesbeth Heyndrickx, Leo Heyndrickx, Katleen Vereecken, Wouter Janssens, Michael S. Seaman, Davide Corti, Antonio Lanzavecchia, David Davis, and Guido Vanham. Characterization of Neutralizing Profiles in HIV-1 Infected Patients from Whom the HJ16, HGN194 and HK20 mAbs Were Obtained. PLoS One, 6(10):e25488, 2011. PubMed ID: 22016769.
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Gonzalez2010
Nuria Gonzalez, Amparo Alvarez, and Jose Alcami. Broadly Neutralizing Antibodies and their Significance for HIV-1 Vaccines. Curr. HIV Res., 8(8):602-612, Dec 2010. PubMed ID: 21054253.
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Lai2012
Rachel P. J. Lai, Michael S. Seaman, Paul Tonks, Frank Wegmann, David J. Seilly, Simon D. W. Frost, Celia C. LaBranche, David C. Montefiori, Antu K. Dey, Indresh K. Srivastava, Quentin Sattentau, Susan W. Barnett, and Jonathan L. Heeney. Mixed Adjuvant Formulations Reveal a New Combination That Elicit Antibody Response Comparable to Freund's Adjuvants. PLoS One, 7(4):e35083, 2012. PubMed ID: 22509385.
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Pantophlet2010
Ralph Pantophlet. Antibody Epitope Exposure and Neutralization of HIV-1. Curr. Pharm. Des., 16(33):3729-3743, 2010. PubMed ID: 21128886.
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Schiffner2016
Torben Schiffner, Natalia de Val, Rebecca A. Russell, Steven W. de Taeye, Alba Torrents de la Peña, Gabriel Ozorowski, Helen J. Kim, Travis Nieusma, Florian Brod, Albert Cupo, Rogier W. Sanders, John P. Moore, Andrew B. Ward, and Quentin J. Sattentau. Chemical Cross-Linking Stabilizes Native-Like HIV-1 Envelope Glycoprotein Trimer Antigens. J. Virol., 90(2):813-828, 28 Oct 2015. PubMed ID: 26512083.
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Sholukh2012
Anton M. Sholukh, Muhammad M. Mukhtar, Michael Humbert, Sosthène S. Essono, Jennifer D. Watkins, Hemant K. Vyas, Vivekanandan Shanmuganathan, Girish Hemashettar, Maria Kahn, Shiu-Lok Hu, David C. Montefiori, Victoria R. Polonis, Peter H. Schur, and Ruth M. Ruprecht. Isolation of Monoclonal Antibodies with Predetermined Conformational Epitope Specificity. PLoS One, 7(6):e38943, 2012. PubMed ID: 22737224.
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Watkins2011a
Jennifer D. Watkins, Nagadenahalli B. Siddappa, Samir K. Lakhashe, Michael Humbert, Anton Sholukh, Girish Hemashettar, Yin Ling Wong, John K. Yoon, Wendy Wang, Francis J. Novembre, Francois Villinger, Chris Ibegbu, Kalpana Patel, Davide Corti, Gloria Agatic, Fabrizia Vanzetta, Siro Bianchi, Jonathan L. Heeney, Federica Sallusto, Antonio Lanzavecchia, and Ruth M. Ruprecht. An Anti-HIV-1 V3 Loop Antibody Fully Protects Cross-Clade and Elicits T-Cell Immunity in Macaques Mucosally Challenged with an R5 Clade C SHIV. PLoS One, 6(3):e18207, 2011. PubMed ID: 21483815.
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