Search Antibody Database

Found 8 matching records:

Displaying record number 15

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MAb ID	B4f8
HXB2 Location	Gag(51-65) DNA(940..984)	Gag Epitope Map
Author Location	p17(51-65)
Epitope	LETSEGCRQILGQLQ	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 1 of 1 note.

• -B4f8: Did not bind live infected cells, only cells that had been made permeable with acetone. Shang1991

References

Showing 1 of 1 reference.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

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Displaying record number 16

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MAb ID	12H-D3b3
HXB2 Location	Gag(62-78) DNA(973..1023)	Gag Epitope Map
Author Location	p17(62-78)
Epitope	GQLQPSLQTGSEELRSL	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 1 of 1 note.

• 12H-D3b3: Did not bind live infected cells, only cells that had been made permeable with acetone. Shang1991

References

Showing 1 of 1 reference.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

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Displaying record number 17

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MAb ID	12G-A8g2
HXB2 Location	Gag(86-115) DNA(1045..1134)	Gag Epitope Map
Author Location	p17(86-115)
Epitope	YCVHQRIEIKDTKEALDKIEEEQNKSKKKA	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 2 of 2 notes.

• 12G-A8g2: This epitope is similar to a fragment of the human protein CD40 ligand TNF-related activation protein (T-cell antigen GP39)(CD154), LDKIEDERN, as well as to a fragment of Lens-epithelium-derived growth factor, DIITEEDKSKKKGQ. Maksiutov2002
• 12G-A8g2: Bound to 30-mer, but not to internal peptides -- did not bind live infected cells -- antigenic domain known as HPG30. Shang1991

References

Showing 2 of 2 references.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

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Displaying record number 18

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MAb ID	12G-D7h11
HXB2 Location	Gag(86-115) DNA(1045..1134)	Gag Epitope Map
Author Location	p17(86-115)
Epitope	YCVHQRIEIKDTKEALDKIEEEQNKSKKKA	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 2 of 2 notes.

• 12G-D7h11: This epitope is similar to a fragment of the human protein CD40 ligand TNF-related activation protein (T-cell antigen GP39) (CD154), LDKIEDERN, as well as to a fragment of Lens-epithelium-derived growth factor, DIITEEDKSKKKGQ. Maksiutov2002
• 12G-D7h11: Bound to 30-mer, but not to internal peptides -- did not bind live infected cells -- antigenic domain known as HPG30. Shang1991

References

Showing 2 of 2 references.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

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Displaying record number 19

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MAb ID	12I-D12g2
HXB2 Location	Gag(86-115) DNA(1045..1134)	Gag Epitope Map
Author Location	p17(86-115)
Epitope	YCVHQRIEIKDTKEALDKIEEEQNKSKKKA	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 2 of 2 notes.

• 12I-D12g2: This epitope is similar to a fragment of the human protein CD40 ligand TNF-related activation protein (T-cell antigen GP39) (CD154), LDKIEDERN, as well as to a fragment of Lens-epithelium-derived growth factor, DIITEEDKSKKKGQ. Maksiutov2002
• 12I-D12g2: Bound to 30-mer, but not to internal peptides -- did not bind live infected cells -- antigenic domain known as HPG30. Shang1991

References

Showing 2 of 2 references.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

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Displaying record number 20

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MAb ID	12G-H1c7
HXB2 Location	Gag(86-115) DNA(1045..1134)	Gag Epitope Map
Author Location	p17(86-115)
Epitope	YCVHQRIEIKDTKEALDKIEEEQNKSKKKA	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	rat(IgG)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	HIV infected-cell lysate
Vaccine strain	B clade IIIB
Vaccine component	HIV-1

Notes

Showing 2 of 2 notes.

• 12G-H1c7: This epitope is similar to a fragment of the human protein CD40 ligand TNF-related activation protein (T-cell antigen GP39) (CD154), LDKIEDERN, as well as to a fragment of Lens-epithelium-derived growth factor, DIITEEDKSKKKGQ. Maksiutov2002
• 12G-H1c7: Bound to 30-mer, but not to internal peptides -- did not bind live infected cells -- antigenic domain known as HPG30. Shang1991

References

Showing 2 of 2 references.

Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

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Displaying record number 36

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MAb ID	G11G1 (12A-Gllgl)
HXB2 Location	Gag	Gag Epitope Map
Author Location	p17
Epitope
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen
Keywords	antibody generation

Notes

Showing 2 of 2 notes.

• Reacted strongly with live cells, but did not possess any measurable virus-neutralizing activities. Shang1991 (antibody generation)
• G11G1: Immunotoxins were generated by linking Env MAbs to ricin A -- immunotoxins mediated cell killing, but only if the antigen was expressed at the cell surface -- ricin-G11G1 did not mediate cell killing. Pincus1996

References

Showing 2 of 2 references.

Isolation Paper
Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

Pincus1996 S. H. Pincus, K. Wehrly, R. Cole, H. Fang, G. K. Lewis, J. McClure, A. J. Conley, B. Wahren, M. R. Posner, A. L. Notkins, S. A. Tilley, A. Pinter, L. Eiden, M. Teintze, D. Dorward, and V. V. Tolstikov. In Vitro Effects of Anti-HIV Immunotoxins Directed against Multiple Epitopes on HIV Type 1 Envelope Glycoprotein 160. AIDS Res. Hum. Retroviruses, 12:1041-1051, 1996. A panel of anti-gp160 MAbs to was used to construct anti-HIV immunotoxins by coupling antibodies to ricin A chain (RAC). The ability of the immunotoxins to kill HIV-1-infected cells was tested in tissue culture. Immunotoxins that bind epitopes on the cell surface killed infected cells, although killing was not directly proportional to binding. The activity of anti-gp41 immunotoxins was markedly enhanced in the presence of sCD4. PubMed ID: 8827220. Show all entries for this paper.

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Displaying record number 38

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MAb ID	G11H3 (12A-Gllh3)
HXB2 Location	Gag	Gag Epitope Map
Author Location	p17
Epitope
Ab Type
Neutralizing
Species (Isotype)	rat(IgG2a)
Patient
Immunogen
Keywords	antibody generation

Notes

Showing 2 of 2 notes.

• Reacted strongly with live cells, but did not possess any measurable virus-neutralizing activities. Shang1991 (antibody generation)
• G11H3: This MAb is cross-reactive between p17 and mycoplasma -- this antibody binds strain specifically to the variable lipoprotein (Vlp) F of M. hyorhinis, in the region of the carboxy-terminal repeat CGGSTPTPEQGNNQGGSTPTPEQGNSQVSK -- the p17 epitope is discontinuous, but p17 and Vlp F share the tetrapeptide SQVS. Pincus1998

References

Showing 2 of 2 references.

Isolation Paper
Shang1991 F. Shang, H. Huang, K. Revesz, H.-C. Chen, R. Herz, and A. Pinter. Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells. J. Virol., 65:4798-4804, 1991. Six MAbs with linear epitopes were mapped. These Abs could only bind to HIV-infected cells that had been permeablized with acetone. Only G11g1 and G11h3, two antibodies that did not bind to peptides, but only to intact p17, could react with live HIV-1 infected cells. These antibodies were not neutralizing. PubMed ID: 1714518. Show all entries for this paper.

Pincus1998 S. H. Pincus, R. L. Cole, R. Watson-McKown, A. Pinter, W. Honnen, B. Cole, and K. S. Wise. Immunologic Cross-Reaction between HIV Type 1 p17 and Mycoplasma hyorhinis Variable Lipoprotein. AIDS Res. Hum. Retroviruses, 14:419-425, 1998. PubMed ID: 9546801. Show all entries for this paper.

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