Search Antibody Database

Found 9 matching records:

Displaying record number 232

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MAb ID	1.2
HXB2 Location	Tat(2-17) DNA(5834..5881)	Tat Epitope Map
Author Location	Tat(1-16)
Epitope	`EPVDPRLEWKHPGSQ`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse
Patient
Immunogen
Keywords

Notes

Showing 1 of 1 note.

1.2: Weak expression of Tat observed in HIV+ brain tissue sample, in contrast to Nef. Ranki1995

References

Showing 2 of 2 reference.

Ovod1992 V. Ovod, A. Lagerstedt, A. Ranki, F. O. Gombert, R. Spohn, M. Tahtinen, G. Jung, and K. J. Krohn. Immunological Variation and Immunohistochemical Localization of HIV-1 Nef Demonstrated with Monoclonal Antibodies. AIDS, 6:25-34, 1992. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 1371924. Show all entries for this paper.

Ranki1995 A. Ranki, M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. Abundant Expression of HIV Nef and Rev Proteins in Brain Astrocytes In Vivo Is Associated with Dementia. AIDS, 9:1001-1008, 1995. HIV Nef protein was found in the brain cells of infected individuals with clinical neurological disease. PubMed ID: 8527071. Show all entries for this paper.

Displaying record number 243

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MAb ID	10.1
HXB2 Location	Rev(33-48) DNA(8399..8446)	Rev Epitope Map
Author Location	Rev(33-48)
Epitope	`GTRQARRNRRRRWRER?`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)
Patient
Immunogen
Keywords

Notes

Showing 2 of 2 notes.

10.1: This epitope is similar to a fragment of the human protein Complement 4 (containing C4A anaphlylatoxin), GRRNRRRR. Maksiutov2002
10.1: Binds to the RRE binding site -- polyclonal anti-Rev Ab detected Rev in astrocytes in 4/5 brain autopsy samples, but only one of these was positive using 10.1, suggesting most Rev was bound to RRE. Ranki1995

References

Showing 4 of 4 references.

Ranki1994 A. Ranki, A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn. Expression Kinetics and Subcellular Localization of HIV-1 Regulatory Proteins Nef and Tat in Established Lymphoid Cell Lines. Arch. Virol., 139:365-378, 1994. PubMed ID: 7832642. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

Displaying record number 917

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MAb ID	3F2
HXB2 Location	Nef(31-40) DNA(8887..8916)	Nef Epitope Map
Author Location	Nef(31-40 BRU)
Epitope	`GAASRDLEKH`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BRU
Vaccine component	Nef

Notes

Showing 4 of 4 notes.

3F2: UK Medical Research Council AIDS reagent: EVA3067.1.
3F2: This epitope is similar to a fragment of the human protein vascular endothelial growth factor C, AEPDAGEATAYASKDLE. Maksiutov2002
3F2: Faintly cross-reactive with astrocytes of uninfected control samples. Ranki1995
3F2: Reacted with Nef from different HIV-1 strains (BRU, IIIB, RF, MN). Ovod1992

References

Showing 4 of 4 references.

Saito1994 Y. Saito, L. Sharer, L. Epstein, and et. al. Overexpression of Nef as a Marker for Restricted HIV-1 Infection of Astrocytes in Postmortem Pediatric Central Nervous Tissues. Neurology, 44:474-481, 1994. PubMed ID: 8145918. Show all entries for this paper.

Displaying record number 918

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MAb ID	3D12
HXB2 Location	Nef(31-50) DNA(8887..8946)	Nef Epitope Map
Author Location	Nef(31-50 BRU)
Epitope	`GAASRDLEKHGAITSSNTAA`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BRU
Vaccine component	Nef

Notes

Showing 6 of 6 notes.

3D12: UK Medical Research Council AIDS reagent: EVA3067.2.
3D12: This epitope is similar to a fragment of the human protein vascular endothelial growth factor C, AEPDAGEATAYASKDLE. Maksiutov2002
3D12: One of four antibodies used in combination to show HIV Nef protein expressed in astrocytes from 7/14 brain samples from HIV+ individuals -- Nef expression associated with dementia. Ranki1995
3D12: Over-expression of Nef in astrocytes from postmortem pediatric CNS tissues. Saito1994
3D12: Reacted with Nef from different HIV-1 strains (BRU, IIIB, RF, MN). Ovod1992
3D12: Database comment: There is an anti-RT MAb that is also named 3D12.

References

Showing 4 of 4 references.

Displaying record number 926

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MAb ID	2F2
HXB2 Location	Nef(151-170) DNA(9247..9306)	Nef Epitope Map
Author Location	Nef(151-170 BRU)
Epitope	`DKVEEANKGENTSLLHPVSL`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Nef

Notes

Showing 6 of 6 notes.

2F2 database note: There are two distinct antibodies named 2F2 in the database.
2F2: UK Medical Research Council AIDS reagent: EVA3067.3.
2F2: This epitope is similar to a fragment of the human protein Hematopoietic progenitor cell antigen CD34, TSLHPVSQHG. Maksiutov2002
2F2: One of four antibodies used in combination to show HIV Nef protein expressed in astrocytes from 7/14 brain samples from HIV+ individuals -- Nef expression associated with dementia. Ranki1995
2F2: Over-expression of Nef in astrocytes from postmortem pediatric CNS tissue. Saito1994
2F2: Strain specific (MN and BRU reactive, not IIIB or RF). Ovod1992

References

Showing 4 of 4 references.

Displaying record number 928

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MAb ID	3E6
HXB2 Location	Nef(161-180) DNA(9277..9336)	Nef Epitope Map
Author Location	Nef(161-180 BRU)
Epitope	`NTSLLHPVSLHGMDDPEREV`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BRU
Vaccine component	Nef

Notes

Showing 4 of 4 notes.

3E6: UK Medical Research Council AIDS reagent: EVA3067.4.
3E6: This epitope is similar to a fragment of the human protein Hematopoietic progenitor cell antigen CD34, TSLHPVSQHG. Maksiutov2002
3E6: Faintly cross-reactive with astrocytes of uninfected control samples. Ranki1995
3E6: Reacted with Nef from different HIV-1 strains (BRU, IIIB, RF, MN). Ovod1992

References

Showing 4 of 4 references.

Displaying record number 929

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MAb ID	3A2
HXB2 Location	Nef(171-190) DNA(9307..9366)	Nef Epitope Map
Author Location	Nef(171-190 BRU)
Epitope	`HGMDDPEREVLEWRFDSRLA`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BRU
Vaccine component	Nef

Notes

Showing 4 of 4 notes.

3A2: UK Medical Research Council AIDS reagent: EVA3067.5.
3A2: One of four antibodies used in combination to show HIV Nef protein expressed in astrocytes from 7/14 brain samples from HIV+ individuals -- Nef expression associated with dementia. Ranki1995
3A2: Over-expression of Nef in astrocytes from postmortem pediatric CNS tissue. Saito1994
3A2: Reacted with Nef from different HIV-1 strains (BRU, IIIB, RF, MN). Ovod1992

References

Showing 3 of 3 references.

Displaying record number 932

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MAb ID	2H12
HXB2 Location	Nef(171-190) DNA(9307..9366)	Nef Epitope Map
Author Location	Nef(171-190 BRU)
Epitope	`HGMDDPEREVLEWRFDSRLA`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BRU
Vaccine component	Nef

Notes

Showing 3 of 3 notes.

2H12: One of four antibodies used in combination to show HIV Nef protein expressed in astrocytes from 7/14 brain samples from HIV+ individuals -- Nef expression associated with dementia. Ranki1995
2H12: Over-expression of Nef in astrocytes from postmortem pediatric CNS tissue. Saito1994
2H12: Reacted with Nef from different HIV-1 strains (BRU, IIIB, RF, MN). Ovod1992

References

Showing 3 of 3 references.

Displaying record number 937

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MAb ID	6.1
HXB2 Location	Nef	Nef Epitope Map
Author Location	Nef( JRCSF)
Epitope	(Discontinuous epitope)
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse
Patient
Immunogen
Keywords	antibody generation

Notes

Showing 2 of 2 notes.

6.1: Raised against CNS primary isolates, stains astrocytes more densely than other Nef MAbs -- Nef expression associated with dementia. Ranki1995 (antibody generation)
6.1: Database comment: there is another mAb in the database with ID 6.1 derived by He2002.

References

Showing 1 of 1 references.

Isolation Paper
Ranki1995 A. Ranki, M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. Abundant Expression of HIV Nef and Rev Proteins in Brain Astrocytes In Vivo Is Associated with Dementia. AIDS, 9:1001-1008, 1995. HIV Nef protein was found in the brain cells of infected individuals with clinical neurological disease. PubMed ID: 8527071. Show all entries for this paper.