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Found 7 matching records:

Displaying record number 1212

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MAb ID	A6
HXB2 Location	Nef(18-26) DNA(8848..8874)	Nef Epitope Map
Author Location	Nef(18-26 NL-432)
Epitope	`ERMRRAEPA?`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgM)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 1 of 1 note.

A6: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. A6 bound to the peptide spanning amino acids 18-26; we inferred the amino acids from the positions in the NL-43 strain. A6 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 1 of 1 reference.

Isolation Paper
Otake1997 Kaori Otake, Yoichi Fujii, Masato Tashiro, Akio Adachi, and Junzoh Kitoh. Epitope Mapping of Murine Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Nef. Exp. Anim., 46(1):53-58, Jan 1997. PubMed ID: 9027472. Show all entries for this paper.

Displaying record number 1213

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MAb ID	A7
HXB2 Location	Nef(28-45) DNA(8878..8931)	Nef Epitope Map
Author Location	Nef(28-45 NL-432)
Epitope	`DGVGAVSRDLEKHGAITS?`	Epitope Alignment
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 1 of 1 note.

A7: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. A7 bound to the peptide spanning amino acids 28-45; we inferred the amino acids from the positions in the NL-43 strain. A7 did not bind to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 1 of 1 reference.

Displaying record number 1216

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MAb ID	F4
HXB2 Location	Nef(115-126) DNA(9139..9174)	Nef Epitope Map
Author Location	Nef(115-126 NL-432)
Epitope	`YHTQGYFPDWQN?`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef

Notes

Showing 2 of 2 notes.

F4: Similarity level of the F4 binding site pentapeptide FPDWQ to the host proteome was low, with the low-similarity 5-mer occurring in the host proteome 0 times, indicating that this peptide can be used to elicit Abs for active/passive immunotherapy with low risk of cross-reaction with the host proteome. Kanduc2008
F4: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. F4 bound to the peptide spanning amino acids 115-126; we inferred the amino acids from the positions in the NL-43 strain. A6 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 2 of 2 references.

Kanduc2008 Darja Kanduc, Rosario Serpico, Alberta Lucchese, and Yehuda Shoenfeld. Correlating Low-Similarity Peptide Sequences and HIV B-Cell Epitopes. Autoimmun. Rev., 7(4):291-296, Feb 2008. PubMed ID: 18295732. Show all entries for this paper.

Displaying record number 1214

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MAb ID	F2
HXB2 Location	Nef(115-136) DNA(9139..9204)	Nef Epitope Map
Author Location	Nef(115-137 NL-432)
Epitope	`YHTQGYFPDWQNYTPGPGVRYP?`	Epitope Alignment
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 1 of 1 note.

F2: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. F2 bound to the peptide spanning amino acids 115-137; we inferred the amino acids from the positions in the NL-43 strain. F2 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 1 of 1 reference.

Displaying record number 1215

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MAb ID	F3
HXB2 Location	Nef(128-137) DNA(9178..9207)	Nef Epitope Map
Author Location	Nef(128-137 NL-432)
Epitope	`TPGPGVRYPL?`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation, complement

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 2 of 2 notes.

F3: Used as a control for Nef binding in a study designed to elicit anti-Nef IgM human Abs in trans-chromosome mice that carry human chromosomes responsible for Ig production. These mice were immunized with recombinant Nef protein. Human heavy chain, mouse light chain anti-Nef IgM were obtained. The hope was that these human IgM anti-Nef chimeric Abs would activate complement mediated cytolysis; C3 deposition was induced but not at high enough levels to induce lysis. Kawai2003 (complement)
F3: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. F3 bound to the peptide spanning amino acids 128-137; we inferred the amino acids from the positions in the NL-43 strain. F3 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 2 of 2 references.

Kawai2003 Masahiro Kawai, Lianying He, Takeshi Kawamura, Shinya Omoto, Yoichi R. Fujii, and Noriko Okada. Chimeric Human/Murine Monoclonal IgM Antibodies to HIV-1 Nef Antigen Expressed on Chronically Infected Cells. Microbiol. Immunol., 47(3):247-253, 2003. PubMed ID: 12725296. Show all entries for this paper.

Displaying record number 1217

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MAb ID	F8
HXB2 Location	Nef(128-137) DNA(9178..9207)	Nef Epitope Map
Author Location	Nef(128-137 NL-432)
Epitope	`TPGPGVRYPL?`	Epitope Alignment
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgM)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 1 of 1 note.

F8: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. F8 bound to the peptide spanning amino acids 128-137; we inferred the amino acids from the positions in the NL-43 strain. F8 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 1 of 1 reference.

Displaying record number 1218

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MAb ID	E5
HXB2 Location	Nef(170-181) DNA(9304..9339)	Nef Epitope Map
Author Location	Nef(170-181)
Epitope	`LHGMDDPEREVL?`	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgM)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade NL43
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Showing 2 of 2 notes.

E5: Similarity level of the E5 binding site pentapeptide GMDDP to the host proteome was low, with the low-similarity 5-mer occurring in the host proteome 1 time, indicating that this peptide can be used to elicit Abs for active/passive immunotherapy with low risk of cross-reaction with the host proteome. Kanduc2008
E5: BALB/c mice were immunized with Nef protein to create a series of anti-Nef antibodies. E5 bound to the peptide spanning amino acids 170-181; we inferred the amino acids from the positions in the NL-43 strain. E5 also bound to the complete Nef protein. Otake1997 (antibody binding site, antibody generation)

References

Showing 2 of 2 references.