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Found 6 matching records:

Displaying record number 236

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MAb ID	4B4C4 (4B4)
HXB2 Location	Tat(49-86) DNA(5975..6045,8379..8421)	Tat Epitope Map
Author Location	Tat
Epitope	RKKRRQRRRPPQGSQTHQVSLSKQPTSQSRGDPTGPKE	Epitope Alignment
Ab Type	C-term
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Tat

Notes

Showing 1 of 1 note.

• 4B4C4: MAb did not bind shorter peptides -- this MAb inhibited exogenously delivered Tat transactivation of an HIV-LTR-CAT construct in HeLa cells by inhibition of cellular uptake of Tat. Valvatne1996

References

Showing 2 of 2 reference.

Valvatne1996 H. Valvatne, A. M. Szilvay, and D. E. Helland. A Monoclonal Antibody Defines a Novel HIV Type 1 Tat Domain Involved in Trans-Cellular Trans-Activation. AIDS Res. Hum. Retroviruses, 12:611-619, 1996. CAT and beta-galactosidase assays, and immunofluorescence analysis were used to study the cellular uptake of the HIV-1 Tat protein. A MAb binding to the basic domain and the RGD sequence inhibits trans- activation by exogenous Tat. The inhibition of the cellular uptake of Tat by an anti-Tat monoclonal antibody and by the serum components implies specific binding of Tat to the cell membrane. PubMed ID: 8743086. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

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Displaying record number 242

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MAb ID	4G9
HXB2 Location	Rev(5-15) DNA(5982..6014)	Rev Epitope Map
Author Location	Rev(5-15)
Epitope	SGDSDEELIRT?	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Rev

Notes

Showing 1 of 1 note.

• 4G9: Mapped binding location by protein footprinting. Jensen1997

References

Showing 1 of 1 reference.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

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Displaying record number 246

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MAb ID	9G2 (9G2G4D6E8)
HXB2 Location	Rev(70-84) DNA(8510..8554)	Rev Epitope Map
Author Location	Rev(70-84)
Research Contact	Anne Marie Szilvay
Epitope	PVPLQLPPLERLTLD	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2aκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Rev

Notes

Showing 4 of 4 notes.

• 9G2: Called 9G2G4D6E8: UK Medical Research Council AIDS reagent: ARP3058.
• 9G2: This epitope is similar fragments of the human protein Epidermal growth factor receptor substrate 15, EPVPMSLPPA, and Insulin-like growth factor binding protein complex acid labile chain precursor, QPPGLERLWLEGNPWDCG. Maksiutov2002
• 9G2: Peptide interaction mapped to aa 70-84, 75-88 -- protein footprint to 65-88. Jensen1997
• 9G2: Worked in indirect immunofluorescence and also detected Rev in WB assays -- used to detect localization of Rev throughout the cell. Kalland1994

References

Showing 3 of 3 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

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Displaying record number 247

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MAb ID	8E7
HXB2 Location	Rev(70-84) DNA(8510..8554)	Rev Epitope Map
Author Location	Rev(70-84)
Epitope	PVPLQLPPLERLTLD	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2aκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Rev

Notes

Showing 4 of 4 notes.

• 8E7: This epitope is similar fragments of the human protein Epidermal growth factor receptor substrate 15, EPVPMSLPPA, and Insulin-like growth factor binding protein complex acid labile chain precursor, QPPGLERLWLEGNPWDCG. Maksiutov2002
• 8E7: HIV-1 RNA and Rev localize to the same region in the nucleoplasm, but the splicing factor SC-35 localizes in different speckles with the nucleoplasm than Rev -- intron containing beta-globin was distributed similarly to HIV-1, suggesting Rev and HIV-1 RNAs interact at putative sites of mRNA transcriptions and splicing. Boe1998
• 8E7: Peptide interaction mapped to aa 70-84, 75-88 -- protein footprint to 65-88. Jensen1997
• 8E7: 8E7 worked in indirect immunofluorescence and also detected Rev in WB assays -- used to detect localization of Rev in several compartments including the nucleoli, nucleoplasm, perinuclear zone, and cytoplasm -- Rev co-localized with host cell factors known to assemble on nascent transcripts -- Rev shuttles continuously between cytoplasmic and nucleoplasmic compartments. Kalland1994,Kalland1994a,Szilvay1995

References

Showing 6 of 6 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Kalland1994a K. H. Kalland, A. M Szilvay, E. Langhoff, and G. Haukenes. Subcellular Distribution of Human Immunodeficiency Virus Type 1 Rev and Colocalization of Rev with RNA Splicing Factors in a Speckled Pattern in the Nucleoplasm. J. Virol., 68:1475-1485, 1994. PubMed ID: 8107211. Show all entries for this paper.

Szilvay1995 A. M. Szilvay, K. A. Brokstad, R. Kopperud, G. Haukenes, and K. H. Kalland. Nuclear Export of the Human Immunodeficiency Virus Type 1 Nucleocytoplasmic Shuttle Protein Rev Is Mediated by Its Activation Domain and Is Blocked by Transdominant Negative Mutants. J. Virol., 69:3315-3323, 1995. PubMed ID: 7745679. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

Boe1998 S. O. Boe, B. Bjorndal, B. Rosok, A. M. Szilvay, and K. H. Kalland. Subcellular Localization of Human Immunodeficiency Virus Type 1 RNAs, Rev, and the Splicing Factor SC-35. Virology, 244:473-482, 1998. PubMed ID: 9601515. Show all entries for this paper.

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.

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Displaying record number 250

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MAb ID	1G10 (IG10F4)
HXB2 Location	Rev(96-105) DNA(8588..8617)	Rev Epitope Map
Author Location	Rev(95-105)
Research Contact	Anne Marie Szilvay
Epitope	GVGSPQILVE	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2bκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Rev

Notes

Showing 4 of 4 notes.

• 1G10: Similarity level of the 1G10 binding site pentapeptide SPQIL to the host proteome was low, with the low-similarity 5-mer occurring in the host proteome 1 time, indicating that this peptide can be used to elicit Abs for active/passive immunotherapy with low risk of cross-reaction with the host proteome. Kanduc2008
• 1G10: Called IG10F4: UK Medical Research Council AIDS reagent: ARP3060.
• 1G10: Peptide interaction mapped to aa 91-105, 96-110 -- protein footprint to aa 10-20, and 95-105. Jensen1997
• 1G10: Bound Rev in indirect immunofluorescence and also detected Rev in WB -- used to detect localization of Rev throughout the cell. Kalland1994

References

Showing 3 of 3 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

Kanduc2008 Darja Kanduc, Rosario Serpico, Alberta Lucchese, and Yehuda Shoenfeld. Correlating Low-Similarity Peptide Sequences and HIV B-Cell Epitopes. Autoimmun. Rev., 7(4):291-296, Feb 2008. PubMed ID: 18295732. Show all entries for this paper.

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Displaying record number 251

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MAb ID	1G7
HXB2 Location	Rev(96-105) DNA(8588..8617)	Rev Epitope Map
Author Location	Rev(95-105)
Epitope	GVGSPQILVE	Epitope Alignment
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG2bκ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Rev

Notes

Showing 2 of 2 notes.

• 1G7: Peptide interaction mapped to aa 91-105, 96-110 -- protein footprint to aa 95-105. Jensen1997
• 1G7: Worked in indirect immunofluorescence and also detected Rev in WB -- used to detect localization of Rev throughout the cell. Kalland1994

References

Showing 2 of 2 references.

Kalland1994 K. H. Kalland, A. M Szilvay, K. A. Brokstad, W. Saetrevik, and G. Haukenes. The Human Immunodeficiency Virus Type 1 Rev Protein Shuttles between the Cytoplasm and Nuclear Compartments. Mol. Cell Biol., 14:7436-7444, 1994. Ten anti-Nef MAbs were generated and mapped. Nef is expressed in two isomorphic forms, and was shown to be expressed mainly in the Golgi complex and at the nuclear membrane, but occasionally x in the nucleus, particularly in MT-4 cells. PubMed ID: 7935458. Show all entries for this paper.

Jensen1997 T. H. Jensen, A. Jensen, A. M. Szilvay, and J. Kjems. Probing the Structure of HIV-1 Rev by Protein Footprinting of Multiple Monoclonal Antibody-Binding Sites. FEBS Lett., 414:50-54, 1997. Rev was mapped using MAb protein footprinting, which gave results that agreed well with peptide mapping, but was useful for identifying a discontinuous interaction between two regions. Footprints supported a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. PubMed ID: 9305730. Show all entries for this paper.

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