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Displaying record number 2890

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MAb ID	CH07
HXB2 Location	Env	Env Epitope Map
Author Location
Epitope
Ab Type	gp120 C5, gp41 fusion domain
Neutralizing	L
Species (Isotype)	human(IgG)
Patient	donor_uncoded_2
Immunogen	HIV-1 infection
Keywords	antibody generation, antibody polyreactivity, mother-to-infant transmission

Notes

Showing 2 of 2 notes.

CH07: A panel of Env-specific mAbs was isolated from 6 HIV1-infected lactating women. Antibodies in colostrum may help prevent mucosal infection of the infant, so this study aimed to define milk IgGs for future vaccination strategies to reduce HIV transmission during lactation. Despite the high rate of VH 1-69 usage among colostrum Env specific B cells, it did not correlate with distinct gp120 epitope specificity or function. CH07 was mentioned as an Ab previously isolated from colostrum; it had significant cross-reactivity with gut bacteria and tested positive in 2 assays for autoreactivity. Jeffries2016 (antibody polyreactivity)
CH07: Two colostrum MAbs CH07 and CH08 were isolated from an HIV-1-infected lactating African woman. They represent the first mucosally-derived anti-HIV MAbs. CH07 binds to linear epitopes in the gp120 C5 region and gp41 fusion domain and weakly neutralized a single tier 1 virus 92TH023. CH07 but not CH08 had strong polyreactivity. Friedman2012 (antibody generation, mother-to-infant transmission, antibody polyreactivity)

References

Showing 2 of 2 references.

Isolation Paper
Friedman2012 James Friedman, S. Munir Alam, Xiaoying Shen, Shi-Mao Xia, Shelley Stewart, Kara Anasti, Justin Pollara, Genevieve G. Fouda, Guang Yang, Garnett Kelsoe, Guido Ferrari, Georgia D. Tomaras, Barton F. Haynes, Hua-Xin Liao, M. Anthony Moody, and Sallie R. Permar. Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum. PLoS One, 7(5):e37648, 2012. PubMed ID: 22624058. Show all entries for this paper.

Jeffries2016 T. L. Jeffries, Jr., C. R. Sacha, J. Pollara, J. Himes, F. H. Jaeger, S. M. Dennison, E. McGuire, E. Kunz, J. A. Eudailey, A. M. Trama, C. LaBranche, G. G. Fouda, K. Wiehe, D. C. Montefiori, B. F. Haynes, H.-X. Liao, G. Ferrari, S. M. Alam, M. A. Moody, and S. R. Permar. The Function and Affinity Maturation of HIV-1 gp120-Specific Monoclonal Antibodies Derived from Colostral B Cells. Mucosal. Immunol., 9(2):414-427, Mar 2016. PubMed ID: 26242599. Show all entries for this paper.

Displaying record number 2891

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MAb ID	CH08
HXB2 Location	Env	Env Epitope Map
Author Location	gp120
Epitope	(Discontinuous epitope)
Ab Type	gp120 CD4i
Neutralizing	P
Species (Isotype)	human(IgG)
Patient	donor_uncoded_2
Immunogen	HIV-1 infection
Keywords	ADCC, antibody generation, antibody polyreactivity, mother-to-infant transmission, neutralization, review, vaccine antigen design, vaccine-induced immune responses

Notes

Showing 4 of 4 notes.

CH08: A panel of Env-specific mAbs was isolated from 6 HIV1-infected lactating women. Antibodies in colostrum may help prevent mucosal infection of the infant, so this study aimed to define milk IgGs for future vaccination strategies to reduce HIV transmission during lactation. Despite the high rate of VH 1-69 usage among colostrum Env specific B cells, it did not correlate with distinct gp120 epitope specificity or function. CH08 was mentioned as an Ab previously isolated from colostrum; it had no significant cross-reactivity with gut bacteria and tested negative in 2 tests of autoreactivity. Jeffries2016 (antibody polyreactivity)
CH08: The complexity of the epitopes recognized by ADCC responses in HIV-1 infected individuals and candidate vaccine recipients is discussed in this review. CH08 is discussed as the CD4i region-targeting, neutralizing anti-gp120 mAb exhibiting ADCC activity and having a discontinuous epitope. Pollara2013 (ADCC, review)
CH08: This review discusses how analysis of infection and vaccine candidate-induced antibodies and their genes may guide vaccine design. This MAb is listed as a cross-reactive neutralizing antibody, with a CD4-inducible epitope. It was isolated after 2009 by FACS of colostrum-derived B cells. Bonsignori2012b (vaccine antigen design, vaccine-induced immune responses, review)
CH08: Two colostrum MAbs CH07 and CH08 were isolated from an HIV-1-infected lactating African woman. They represent the first mucosally-derived anti-HIV MAbs. CH08 recognized a CD4i epitope and had moderate breadth of neutralization by neutralizing multipe tier 1 and tier 2 viruses in a pattern similar to that of CD4i MAbs 17b and 412-D. Unlike CH07, CH08 was nonpolyreactive. Friedman2012 (ADCC, antibody generation, neutralization, mother-to-infant transmission)

References

Showing 4 of 4 references.

Bonsignori2012b Mattia Bonsignori, S. Munir Alam, Hua-Xin Liao, Laurent Verkoczy, Georgia D. Tomaras, Barton F. Haynes, and M. Anthony Moody. HIV-1 Antibodies from Infection and Vaccination: Insights for Guiding Vaccine Design. Trends Microbiol., 20(11):532-539, Nov 2012. PubMed ID: 22981828. Show all entries for this paper.

Pollara2013 Justin Pollara, Mattia Bonsignori, M. Anthony Moody, Marzena Pazgier, Barton F. Haynes, and Guido Ferrari. Epitope Specificity of Human Immunodeficiency Virus-1 Antibody Dependent Cellular Cytotoxicity (ADCC) Responses. Curr. HIV Res., 11(5):378-387, Jul 2013. PubMed ID: 24191939. Show all entries for this paper.