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Displaying record number 869

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MAb ID	D43
HXB2 Location	Env	Env Epitope Map
Author Location	gp41( HXB2)
Research Contact	Patricia Earl and Christopher Broder, NIH
Epitope
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Env dimers

Notes

Showing 3 of 3 notes.

D43: Partially conformation dependent -- doesn't bind to short peptides, but does bind to the region spanning 641-683 -- binding can be blocked by MAbs T3, D38 and D45 -- MAbs in this competition group reacted with 9/10 HIV-1 strains, not binding to JRFL. Earl1997
D43: This is a linear gp41 epitope, mapping in the region 635-678 -- human sera blocked binding in oligomeric ELISA assay to a similar extent for gp41 MAbs D20, D43, D61, and T4. Richardson1996
D43: Generated during a study of the influence of the oligomeric structure of Env in determining the repertoire of the Ab response. Earl1994

References

Showing 3 of 3 references.

Earl1994 P. L. Earl, C. C. Broder, D. Long, S. A. Lee, J. Peterson, S. Chakrabarti, R. W. Doms, and B. Moss. Native oligomeric human immunodeficiency virus type 1 Envelope glycoprotein elicits diverse monoclonal antibody reactivities. J. Virol., 68:3015-3026, 1994. In a study of the repertoire of response to oligomeric versus monomeric Env protein, 138 murine MAbs were generated in response to an immunogen that was a gp120/bp41 oligomeric molecule that was not cleaved due to a mutation in the cleavage site. The oligomeric molecule was found to elicit a response that was very different than the monomer. Most MAbs were conformational, many were to gp41 or if in gp120, to the CD4 BS. Few MAbs to linear V3 epitopes were produced in response to oligomeric protein, though this was a common specificity in response to immunization with gp120 monomeric protein. PubMed ID: 7512157. Show all entries for this paper.

Richardson1996 T. M. Richardson, Jr., B. L. Stryjewski, C. C. Broder, J. A. Hoxie, J. R. Mascola, P. L. Earl, and R. W. Doms. Humoral response to oligomeric human immunodeficiency virus type 1 envelope protein. J. Virol., 70:753-62, 1996. An Env antigen capture enzyme-linked immunosorbent assay using a soluble, oligomeric form of HIV-1IIIB Env (gp140) that contains gp120 and the gp41 ectodomain was developed. The gp140, captured by various monoclonal antibodies (MAbs), retained its native oligomeric structure: it bound CD4 and was recognized by MAbs to conformational epitopes in gp120 and gp41, including oligomer-specific epitopes in gp41. PubMed ID: 8551612. Show all entries for this paper.

Earl1997 P. L. Earl, C. C. Broder, R. W. Doms, and B. Moss. Epitope map of human immunodeficiency virus type 1 gp41 derived from 47 monoclonal antibodies produced by immunization with oligomeric envelope protein. J. Virol., 71:2674-84, 1997. PubMed ID: 9060620. Show all entries for this paper.