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Displaying record number 867

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MAb ID	D16
HXB2 Location	Env	Env Epitope Map
Author Location	gp41( IIIB)
Research Contact	Patricia Earl and Christopher Broder, NIH
Epitope
Ab Type
Neutralizing	L
Species (Isotype)	mouse(IgG)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Env dimers

Notes

Showing 3 of 3 notes.

D16: One of eleven MAbs (D16, D17, D31, D36, D37, D40, D44, D55, D59, T37, and T45) that are conformation dependent and that can block the binding of the MAb D50 that binds to the linear peptide gp41(642-665) -- reactive with 9/10 HIV-1 strains all except HIV-1 ADA, which has the change E659D and E662A that may result in the loss of binding (ELLE to DLLA). Earl1997
D16: Precipitates both oligomeric gp140 and soluble monomeric gp41(21-166)that lacks the fusion peptide and membrane anchor, along with MAbs D16, D38, D40, D41, and D54. Weissenhorn1996
D16: Generated during a study of the influence of the oligomeric structure of Env in determining the repertoire of the Ab response. Earl1994

References

Showing 3 of 3 references.

Earl1994 P. L. Earl, C. C. Broder, D. Long, S. A. Lee, J. Peterson, S. Chakrabarti, R. W. Doms, and B. Moss. Native oligomeric human immunodeficiency virus type 1 Envelope glycoprotein elicits diverse monoclonal antibody reactivities. J. Virol., 68:3015-3026, 1994. In a study of the repertoire of response to oligomeric versus monomeric Env protein, 138 murine MAbs were generated in response to an immunogen that was a gp120/bp41 oligomeric molecule that was not cleaved due to a mutation in the cleavage site. The oligomeric molecule was found to elicit a response that was very different than the monomer. Most MAbs were conformational, many were to gp41 or if in gp120, to the CD4 BS. Few MAbs to linear V3 epitopes were produced in response to oligomeric protein, though this was a common specificity in response to immunization with gp120 monomeric protein. PubMed ID: 7512157. Show all entries for this paper.

Weissenhorn1996 W. Weissenhorn, S. A. Wharton, L. J. Calder, P. L. Earl, B. Moss, E. Aliprandis, J. J. Skehel, and D. C. Wiley. The Ectodomain of HIV-1 Env Subunit gp41 Forms a Soluble, alpha-Helical, Rod-Like Oligomer in the Absence of gp120 and the N-Terminal Fusion Peptide. EMBO J., 15:1507-1514, 1996. PubMed ID: 8612573. Show all entries for this paper.

Earl1997 P. L. Earl, C. C. Broder, R. W. Doms, and B. Moss. Epitope map of human immunodeficiency virus type 1 gp41 derived from 47 monoclonal antibodies produced by immunization with oligomeric envelope protein. J. Virol., 71:2674-84, 1997. PubMed ID: 9060620. Show all entries for this paper.