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Displaying record number 677

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MAb ID	ID6
HXB2 Location	gp160(87-101) DNA(6483..6527)	gp160 Epitope Map
Author Location	gp120(86-100 LAV)
Epitope	`VNVTENFNMWKNNMV` (Discontinuous epitope)	Epitope Alignment
Subtype	B
Ab Type	N-term
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation, antibody interactions

Vaccine Details

Vaccine type	protein
Vaccine component	gp160

Notes

Showing 6 of 6 notes.

ID6: Ugen1993 is cited by Cook1994 as the source of the MAb. Dickey2000 later published the details of the MAb isolation. Ugen1993 and Dickey2000 have approximately the same set of authors, thus this is not a case of two antibodies with the same name. Ugen1993
ID6: Used phage display to define the epitope of ID6 to gp120 residues 86-100 (HIV-MN). Gomez-Roman2002 (antibody binding site)
ID6: Antibody source: tissue culture supernatant; obtained from the NIH AIDS Research and Reference Reagent Program. Catalog # 2343. The capacity of ID6 to block completely the activity of the anti-HIV peptide T20 was investigated. T20 inhibited the fusion or syncytia formation between co-cultured CHO-WT cells expressing HIV-1 HXB2 envelope glycoprotein on their surface and HeLaT4 cells. ID6 blocked the anti-fusion effect of T20. Vincent2012 (antibody interactions)
ID6: NIH AIDS Research and Reference Reagent Program: 2343.
ID6: MAbs against the glycosphingolipid GalCer block HIV infection of normally susceptible CD4 negative cells from the brain and colon -- MAbs against the N-terminal half of gp120 do not inhibit gp120 binding to GalCer -- binding of GalCer to gp120 does not inhibit MAb binding. Cook1994 (antibody interactions)
ID6: Three MABs, ID6, AC4, and AD3 that bind to a discontinuous N-term first 204 aa of gp120 and generate ADCC were elicited through vaccination of BALBc mice with rec gp160 -- these MAbs do not depend on glycosylation and are cross-reactive with viruses from clades B and CRF01(AE). Dickey2000 (antibody generation)

References

Showing 5 of 5 references.

Isolation Paper
Dickey2000 C. Dickey, U. Ziegner, M. G. Agadjanyan, V. Srikantan, Y. Refaeli, A. Prabhu, A. Sato, W. V. Williams, D. B. Weiner, and K. E. Ugen. Murine Monoclonal Antibodies Biologically Active against the Amino Region of HIV-1 gp120: Isolation and Characterization. DNA Cell Biol., 19:243-252, 2000. PubMed ID: 10798448. Show all entries for this paper.

Cook1994 D. G. Cook, J. Fantini, S. L. Spitalnik, and F. Gonzalez-Scarano. Binding of Human Immunodeficiency Virus Type 1 HIV-1 gp120 to Galactosylceramide (GalCer): Relationship to the V3 Loop. Virol., 201:206-214, 1994. Antibodies against GalCer can block infection of CD4-negative cells from the brain and colon that are susceptible to HIV infection. This paper explores the ability of a panel of MAbs to inhibit binding of gp120 to GalCer, and also of the binding of GalCer to inhibit MAb-gp120 interaction. MAbs to the V3 loop and GalCer showed mutual inhibition of binding to gp120, and anti-CD4 binding site MAbs showed reduced inhibition. N- and C-terminal MAbs didn't influence GalCer binding. PubMed ID: 8184533. Show all entries for this paper.

Gomez-Roman2002 Victor Raul Gómez-Román, Chuanhai Cao, Yun Bai, Hugo Santamaría, Gonzalo Acero, Karen Manoutcharian, David B Weiner, Kenneth E Ugen, and Goar Gevorkian. Phage-Displayed Mimotopes Recognizing a Biologically Active Anti-HIV-1 gp120 Murine Monoclonal Antibody. J. Acquir. Immune Defic. Syndr., 31(2):147-153, 1 Oct 2002. PubMed ID: 12394792. Show all entries for this paper.

Ugen1993 K. E. Ugen, Y. Refaleli, U. Ziegner, M. Agadjanyan, M. A. R. Satre, V. Srikantan, B. Wang, A. Sato, W. V. Williams, and D. B. Weiner. Generation of monoclonal antibodies against the amino terminus of gp120 that elicit antibody-dependent cellular cytotoxicity. Vaccines 93, :215-221, 1993. Editors: F. Brown, H. S. Ginsberg and R. Lerner, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Show all entries for this paper.

Vincent2012 Nadine Vincent and Etienne Malvoisin. Ability of Antibodies Specific to the HIV-1 Envelope Glycoprotein to Block the Fusion Inhibitor T20 in a Cell-Cell Fusion Assay. Immunobiology, 217(10):943-950, Oct 2012. PubMed ID: 22387075. Show all entries for this paper.