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Displaying record number 651

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MAb ID	MAG 96
HXB2 Location	Env	Env Epitope Map
Author Location	gp120
Research Contact	C. Y. Kang, IDEC Inc
Epitope
Ab Type	gp120 CD4BS
Neutralizing	L
Species (Isotype)	mouse
Patient
Immunogen	vaccine
Keywords	antibody binding site

Vaccine Details

Vaccine type	sCD4-gp120 complex
Vaccine strain	B clade HXB2
Vaccine component	gp120

Notes

Showing 2 of 2 notes.

MAG 96: IgG antibody phage display libraries were created from HIV-1+ individuals after pre-selection of PBMC with gp120, as an alternative to using bone marrow for generating libraries. MAG 96 was among a set of Abs used for competition studies to define the binding sites of the newly isolated MAbs, representing a MAb with a CD4BS epitope. Koefoed2005 (antibody binding site)
MAG 96: Amino acid substitutions that reduce binding 10 fold: 256 S/Y, 257 T/R, 368 D/R or T, 370 E/R -- weak neutralization of IIIB. Kang1994

References

Showing 2 of 2 references.

Kang1994 C.-Y. Kang, K. Hariharan, P. L. Nara, J. Sodroski, and J. P. Moore. Immunization with a Soluble CD4-gp120 Complex Preferentially Induces Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibodies Directed to Conformation-Dependent Epitopes of gp120. J. Virol., 68:5854-5862, 1994. Most of the MAbs generated in this study were conformational, but there were four that bound a V3 loop peptide. These four could neutralize lab strains with different efficiencies. These MAbs were very sensitive to substitutions in the V3 loop, but also to substitutions in the base of the V1/V2 loop structure (120/121 VK/LE), indicating an underlying conformational character. Additionally, many anti-CD4 binding site MAbs were described, that shared a sensitivity to substitutions at residues 368 and 370. Another class of MAbs was found that appeared to be conformationally sensitive, and shared a reduction in binding with the amino acid substitution 88 N/P in the C1 domain. PubMed ID: 7520095. Show all entries for this paper.

Koefoed2005 Klaus Koefoed, Lauge Farnaes, Meng Wang, Arne Svejgaard, Dennis R. Burton, and Henrik J. Ditzel. Molecular Characterization of the Circulating Anti-HIV-1 gp120-Specific B Cell Repertoire using Antibody Phage Display Libraries Generated from Pre-Selected HIV-1 gp120 Binding PBLs. J. Immunol. Methods, 297(1-2):187-201, Feb 2005. PubMed ID: 15777942. Show all entries for this paper.