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Displaying record number 649

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MAb ID	MAG 72 (L72)
HXB2 Location	Env	Env Epitope Map
Author Location	gp120
Research Contact	C. Y. Kang or Dr. Hariharam, IDEC Pharmaceuticals Corp, La Jolla, CA
Epitope
Ab Type	gp120 CD4BS
Neutralizing	L
Species (Isotype)	mouse
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	sCD4-gp120 complex
Vaccine strain	B clade HXB2
Vaccine component	gp120

Notes

Showing 2 of 2 notes.

MAG 72: Called L72 -- used to bind gp120 to solid phase to select MAbs from a phage selection library. Ditzel1997
MAG 72: Amino acid substitutions that reduce binding 10 fold: 257 T/R or A or G, 262 N/T, 368 D/R or T, 370 E/R or Q, 384 Y/E, 421 K/L, 477 D/V -- neutralizes MN, IIIB and RF. Kang1994

References

Showing 2 of 2 references.

Kang1994 C.-Y. Kang, K. Hariharan, P. L. Nara, J. Sodroski, and J. P. Moore. Immunization with a Soluble CD4-gp120 Complex Preferentially Induces Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibodies Directed to Conformation-Dependent Epitopes of gp120. J. Virol., 68:5854-5862, 1994. Most of the MAbs generated in this study were conformational, but there were four that bound a V3 loop peptide. These four could neutralize lab strains with different efficiencies. These MAbs were very sensitive to substitutions in the V3 loop, but also to substitutions in the base of the V1/V2 loop structure (120/121 VK/LE), indicating an underlying conformational character. Additionally, many anti-CD4 binding site MAbs were described, that shared a sensitivity to substitutions at residues 368 and 370. Another class of MAbs was found that appeared to be conformationally sensitive, and shared a reduction in binding with the amino acid substitution 88 N/P in the C1 domain. PubMed ID: 7520095. Show all entries for this paper.

Ditzel1997 H. J. Ditzel, P. W. Parren, J. M. Binley, J. Sodroski, J. P. Moore, C. F. Barbas, III, and D. R. Burton. Mapping the Protein Surface of Human Immunodeficiency Virus Type 1 gp120 Using Human Monoclonal Antibodies from Phage Display Libraries. J. Mol. Biol., 267:684-695, 1997. (Genbank: U82767 U82768 U82769 U82770 U82771 U82772 U82942 U82943 U82944 U82945 U82946 U82947 U82948 U82949 U82950 U82951 U82952 U82961 U82962) Recombinant monoclonal antibodies from phage display libraries provide a method for Env surface epitope mapping. Diverse epitopes are accessed by presenting gp120 to the library in different forms, such as sequential masking of epitopes with existing MAbs or sCD4 prior to selection or by selection on peptides. Fabs identified by these methods have specificities associated with epitopes presented poorly on native multimeric envelope. PubMed ID: 9126846. Show all entries for this paper.