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Displaying record number 607

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MAb ID	558-D
HXB2 Location	Env	Env Epitope Map
Author Location	gp120
Research Contact	Susan Zolla-Pazner (Zollas01@mcrcr6.med.nyu), NYU Med Center, NY, NY
Epitope
Ab Type	gp120 CD4BS
Neutralizing	L
Species (Isotype)	human
Patient
Immunogen	HIV-1 infection
Keywords	antibody binding site, review, subtype comparisons, variant cross-reactivity

Notes

Showing 3 of 3 notes.

558-D: This review summarizes MAbs directed to HIV-1 Env. There are 51 CD4BS MAbs and Fabs in the database; most, like this MAb, neutralize TCLA strains only. Gorny2003 (review)
558-D: Using a whole virion-ELISA method, 18 human MAbs were tested for their ability to bind to a panel of 9 viruses from clades A, B, D, F, G, and H -- CD4BS Abs tended to bind weakly without clade specificity to virions, but bound well to soluble gp120 -- 558-D did not bind to any B clade viruses, and weakly bound to clade A, C, and G isolates -- 559/64-D, 558-D and 1202-D had similar reactivities. Nyambi1998 (variant cross-reactivity, subtype comparisons)
558-D: Blocks gp120-CD4 binding -- binds a panel of mutants all except for 256 S/Y and 262 N/T, which are probably conformationally disruptive. McKeating1992b (antibody binding site)

References

Showing 3 of 3 references.

McKeating1992b J. A. McKeating, M. Thali, C. Furman, S. Karwowska, M. K. Gorny, J. Cordell, S. Zolla-Pazner, J. Sodroski, and R. A. Weiss. Amino Acid Residues of the Human Immunodeficiency Virus Type 1 gp120 Critical for the Binding of Rat and Human Neutralizing Antibodies That Block the gp120-sCD4 Interaction. Virology, 190:134-142, 1992. PubMed ID: 1382339. Show all entries for this paper.

Nyambi1998 P. N. Nyambi, M. K. Gorny, L. Bastiani, G. van der Groen, C. Williams, and S. Zolla-Pazner. Mapping of Epitopes Exposed on Intact Human Immunodeficiency Virus Type 1 (HIV-1) Virions: A New Strategy for Studying the Immunologic Relatedness of HIV-1. J. Virol., 72:9384-9391, 1998. 18 human MAbs binding to gp120 and gp41 were tested using a novel assay to test binding to intact HIV-1 virions. The new method involves using MAbs to the host proteins incorporated into virions to bind them to ELIZA plates. Antigenic conservation in epitopes of HIV-1 in clades A, B, D, F, G, and H was studied. MAbs were selected that were directed against V2, V3, CD4bd, C5 or gp41 regions. Antibodies against V2, the CD4BS, and sp41 showed weak and sporadic reactivities, while binding strongly to gp120, suggesting these epitopes are hidden when gp120 is in its native, quaternary structure. PubMed ID: 9765494. Show all entries for this paper.

Gorny2003 Miroslaw K. Gorny and Susan Zolla-Pazner. Human Monoclonal Antibodies that Neutralize HIV-1. In Bette T. M. Korber and et. al., editors, HIV Immunology and HIV/SIV Vaccine Databases 2003. pages 37--51. Los Alamos National Laboratory, Theoretical Biology \& Biophysics, Los Alamos, N.M., 2004. URL: http://www.hiv.lanl.gov/content/immunology/pdf/2003/zolla-pazner_article.pdf. LA-UR 04-8162. Show all entries for this paper.