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Displaying record number 520

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MAb ID	B6
HXB2 Location	Env	Env Epitope Map
Author Location	gp120( IIIB)
Epitope
Ab Type
Neutralizing
Species (Isotype)	mouse(IgM)
Patient
Immunogen	vaccine
Keywords	antibody generation, antibody sequence, autoantibody or autoimmunity, binding affinity, vaccine antigen design

Vaccine Details

Vaccine type	chimeric GM-CSF
Vaccine strain	B clade IIIB
Vaccine component	gp120

Notes

Showing 2 of 2 notes.

B6: Lipid-based nanoparticles for the multivalent display of trimers have been shown to enhance humoral responses to trimer immunogens in the context of HIV vaccine development. After immunization with soluble MD39 SOSIP trimers (a stabilized version of BG505), trimer-conjugated liposomes improved both germinal center B cell and trimer-specific T follicular helper cell responses. In particular, MD39-liposomes showed high levels of binding by bNAbs such as V3 glycan specific PGT121, V1/V2 glycan specific PGT145, gp120/gp41 interface specific PGT151, CD4 binding site specific VRC01, and showed minimal binding by non-NAbs like CD4 binding site specific B6, and V3 specific 4025 or 39F. Tokatlian2018 (vaccine antigen design, binding affinity)
B6: Murine antibody response to the chimeric construction granulocyte-macrophage colony stimulating factor GM-CSF/gp120 was tested, MAbs from normal mice were gp120 specific, MAbs from nude mice bound gp120 but were polyreactive, and from reconstituted mice were half way between -- the anti-gp120 response used a high frequency of VH81X, VHQ52, and VH7183 genes, a family used during fetal life and associated with autoimmunity -- B6 was a gp120 from a BALBc reconstructed nude mouse and had VH gene J558. delReal1999 (antibody generation, autoantibody or autoimmunity, antibody sequence)

References

Showing 2 of 2 references.

Isolation Paper
delReal1999 G. del Real, M. Llorente, P. Lucas, L. Kremer, J. L. Toran, and Martinez-A C. Antibody Repertoire against HIV-1 gp120 Triggered in Nude and Normal Mice by GM-CSF/gp120 Immunization. Mol. Immunol., 36:721-731, 1999. PubMed ID: 10593511. Show all entries for this paper.

Tokatlian2018 Talar Tokatlian, Daniel W. Kulp, Andrew A. Mutafyan, Christopher A. Jones, Sergey Menis, Erik Georgeson, Mike Kubitz, Michael H. Zhang, Mariane B. Melo, Murillo Silva, Dong Soo Yun, William R. Schief, and Darrell J. Irvine. Enhancing Humoral Responses Against HIV Envelope Trimers via Nanoparticle Delivery with Stabilized Synthetic Liposomes. Sci. Rep., 8(1):16527, 8 Nov 2018. PubMed ID: 30410003. Show all entries for this paper.