Search Antibody Database

Found 1 matching record:

Displaying record number 4145

Download this epitope record as JSON.

MAb ID	12G10
HXB2 Location	Env	Env Epitope Map
Author Location	gp120
Epitope
Subtype	B
Ab Type	gp120 CD4i
Neutralizing	L
Species (Isotype)	human(IgG)
Patient	KTS376
Immunogen	HIV-1 infection
Country	Japan
Keywords	antibody binding site, antibody generation, neutralization

Notes

Showing 2 of 2 notes.

12G10: Database note: There are 2 different mAbs named 12G10 in the database.
12G10: Two CD4i mAbs were derived from donor KTS376 (25C4b and 12G10), in addition to those previously isolated by RamirezValdez2015. Single chain variable fragments (scFvs) were constructed for three CD4i-targeting mAbs from donor KTS376: 916B2, 4E9C, and 25C4b. Coverage of neutralization by the scFvs against a panel of 66 multiclade pseudoviruses was 89% for 4E9C, 95% for 25C4b, and 100% for 916B2. 25C4b bound the region spanning multiple domains of hairpin 1 (H1) and H2 of the bridging sheet and V3 base, similar to mAb 17b. For 4E9C, V3-base dependent binding was apparent based on lack of binding to mutants containing a V3 truncation. In contrast, binding of 916B2 was dependent on the H1 region. It was noted that 4E9C and 12G10 belong to the same clonal lineage. Tanaka2017 (antibody binding site, antibody generation, neutralization)

References

Showing 1 of 1 references.

Isolation Paper
Tanaka2017 Kazuki Tanaka, Takeo Kuwata, Muntasir Alam, Gilad Kaplan, Shokichi Takahama, Kristel Paola Ramirez Valdez, Anna Roitburd-Berman, Jonathan M. Gershoni, and Shuzo Matsushita. Unique binding modes for the broad neutralizing activity of single-chain variable fragments (scFv) targeting CD4-induced epitopes. Retrovirology, 14(1):44 doi, Sep 2017. PubMed ID: 28938888 Show all entries for this paper.