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Displaying record number 3989

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MAb ID	10E8/PGT121/PGDM1400.V8Fc (10E8Fab-PGT121fv-PGDM1400fv.V8Fc)
HXB2 Location	Env	Env Epitope Map
Author Location
Epitope
Ab Type	gp120 V2 // V2 glycan(V2g) // V2 apex, gp120 V3 // V3 glycan (V3g), gp41 MPER (membrane proximal external region)
Neutralizing	P (tier 2) View neutralization details
Species (Isotype)
Patient	Donor 17, Donor 84, Donor N152
Immunogen	engineered
Keywords	antibody generation, bispecific/trispecific, neutralization

Notes

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10E8/PGT121/PGDM1400.V8Fc: The authors describe single-component molecules they designed that incorporate two (bispecific) or three (trispecific) bNAbs that recognize HIV Env exclusively, a bispecific CrossmAb targeting two epitopes on the major HIV coreceptor, CCR5, and bi- and trispecifics that cross-target both Env and CCR5. These newly designed molecules displayed exceptional breadth, neutralizing 98 to 100% of a 109-virus panel, as well as additivity and potency compared to those of the individual parental control IgGs. They constructed 8 different versions of tri-specific 10E8Fab-PGT121fv-PGDM1400fv, with the three bNAb-based specificities in different orientations, of which V8 was 99% effective against a panel of 109 viruses. Therefore, 3 new variants out of the V8 variant were constructed. The 10E8Fab-PGT121fv-PGDM1400fv.V8Fc trispecific (Fc region incorporated for binding to FcRn) retained a comparable neutralizing capacity and, in some cases, demonstrated enhanced neutralization potency against isolates not readily neutralized by the original V8 variant. Khan2018 (antibody generation, neutralization, bispecific/trispecific)

References

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Khan2018 Salar N. Khan, Devin Sok, Karen Tran, Arlette Movsesyan, Viktoriya Dubrovskaya, Dennis R. Burton, and Richard T. Wyatt. Targeting the HIV-1 Spike and Coreceptor with Bi- and Trispecific Antibodies for Single-Component Broad Inhibition of Entry. J. Virol., 92(18), 15 Sep 2018. PubMed ID: 29976677. Show all entries for this paper.