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MAb ID	DH517
HXB2 Location	Env	Env Epitope Map
Author Location	gp41
Epitope
Ab Type	gp41 MPER (membrane proximal external region)
Neutralizing	P (tier 2) View neutralization details
Species (Isotype)	human(IgG)
Patient	CH0210
Immunogen	HIV-1 infection
Keywords	antibody generation, neutralization

Notes

Showing 3 of 3 notes.

DH517: Plasma from donor PG13 was found to have MPER neutralization activity, and mAb PGZL1 was isolated. When compared to a 4E10, PGZL1 was found to share similar crystal structure, contacts, and some common germline genes, but its neutralization and polyreactivity were less strong. The germline gene usage of PGZL1 was compared with other MPER antibodies: 4E10, VRC42.01, 10E8, DH511.2, DH517, Z13, and 2F5. Zhang2019a
DH517: Six clonally related mAbs, designated DH511.1 to DH511.6 were isolated by single memory B cell sorting from a clade-C-infected African donor, CH0201. Among them, DH511.2 had the most potent neutralization. Several additional antibodies were derived from the patient's plasma and designated DH511.7P - DH511.12P. In a panel of 208 HIV-1 isolates, DH511.11P and DH511.12P had slightly increased breadth and potency compared with DH511.2. Thirty-five chimeric mAbs were produced by swapping the heavy and light chains of DH511.2 with those of plasma DH511 lineage members; among these, DH511.2-K3 had the highest potency. A second lineage, represented by DH517, was also isolated from the same patient. Its neutralization was less potent than DH511.2. Williams2017 (antibody generation, neutralization)
DH517: This patent application describes MPER antibody lineages DH511, DH512, DH513, DH514, DH515, DH516, DH517, DH518, DH536, DH537, DH491, DH493 and derivative antibodies. Antibodies DH511-517 were derived from donor CH0210. Haynes2016a

References

Showing 3 of 3 references.

Isolation Paper
Williams2017 LaTonya D. Williams, Gilad Ofek, Sebastian Schätzle, Jonathan R. McDaniel, Xiaozhi Lu, Nathan I. Nicely, Liming Wu, Caleb S. Lougheed, Todd Bradley, Mark K. Louder, Krisha McKee, Robert T. Bailer, Sijy O'Dell, Ivelin S. Georgiev, Michael S. Seaman, Robert J. Parks, Dawn J. Marshall, Kara Anasti, Guang Yang, Xiaoyan Nie, Nancy L. Tumba, Kevin Wiehe, Kshitij Wagh, Bette Korber, Thomas B. Kepler, S. Munir Alam, Lynn Morris, Gift Kamanga, Myron S. Cohen, Mattia Bonsignori, Shi-Mao Xia, David C. Montefiori, Garnett Kelsoe, Feng Gao, John R. Mascola, M. Anthony Moody, Kevin O. Saunders, Hua-Xin Liao, Georgia D. Tomaras, George Georgiou, and Barton F. Haynes. Potent and Broad HIV-Neutralizing Antibodies in Memory B Cells and Plasma. Sci. Immunol., 2(7), 27 Jan 2017. PubMed ID: 28783671. Show all entries for this paper.

Haynes2016a B. F. Haynes, H. X. Liao, M. A. Moody, L. Williams, K. J. Wiehe, and G. A. Ofek. HIV-1 Neutralizing Antibodies and Uses Thereof. WIPO patent application, Sep 2016. URL: https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2016149710. Application number PCT/US2016/023488. Show all entries for this paper.

Zhang2019a Lei Zhang, Adriana Irimia, Lingling He, Elise Landais, Kimmo Rantalainen, Daniel P. Leaman, Thomas Vollbrecht, Armando Stano, Daniel I. Sands, Arthur S. Kim, IAVI Protocol G Investigators, Pascal Poignard, Dennis R. Burton, Ben Murrell, Andrew B. Ward, Jiang Zhu, Ian A. Wilson, and Michael B. Zwick. An MPER Antibody Neutralizes HIV-1 Using Germline Features Shared Among Donors. Nat. Commun., 10(1):5389, 26 Nov 2019. PubMed ID: 31772165. Show all entries for this paper.