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Displaying record number 3393

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MAb ID	PCDN-22A (22A)
HXB2 Location	Env	Env Epitope Map
Author Location	Env
Epitope
Subtype	C
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing	P (tier 2)
Contacts and Features	View contacts and features
Species (Isotype)	human
Patient	PC76
Immunogen	HIV-1 infection
Keywords	antibody generation, antibody lineage, mutation acquisition, neutralization, structure

Notes

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22A: For the first time, a lineage of 12 bnAbs targeting the Env high-mannose patch were isolated from donor PC76. All mAbs exhibited binding to wild type gp120 and reduced binding to N332A gp120. Neutralization breadth increased for Abs isolated from later timepoints, with 33A, 38A and 38B being the most mature. This was due to a combination of mutations arising in autologous virus, specifically E328K/A328K and deletion of glycosylation site N335, a unique feature of PC76 autologous virus, that increased viral sensitivity to NAb. The final route of escape however, was elimination of a glycan site at HIV-1 N332 or N301. Ab 22A was isolated from serum derived at 22 months post-infection. It neutralized 13.5% of a 37-virus neutralization panel at IC₅₀ geometric mean of 15.49 µg/ml. MacLeod2016 (antibody generation, mutation acquisition, neutralization, structure, antibody lineage)

References

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Isolation Paper
MacLeod2016 Daniel T. MacLeod, Nancy M Choi, Bryan Briney, Fernando Garces, Lorena S. Ver, Elise Landais, Ben Murrell, Terri Wrin, William Kilembe, Chi-Hui Liang, Alejandra Ramos, Chaoran B Bian, Lalinda Wickramasinghe, Leopold Kong, Kemal Eren, Chung-Yi Wu, Chi-Huey Wong, IAVI Protocol C Investigators and The IAVI African HIV Research Network, Sergei L. Kosakovsky-Pond, Ian A. Wilson, Dennis R. Burton, and Pascal Poignard. Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch. Immunity, 44(5):1215-1226, 17 May 2016. PubMed ID: 27192579. Show all entries for this paper.