Search Antibody Database

Found 1 matching record:

Displaying record number 3389

Download this epitope record as JSON.

MAb ID	PCDN-38A (38A, PCDN38A)
HXB2 Location	Env	Env Epitope Map
Author Location	Env
Epitope
Subtype	C
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing	P (tier 2) View neutralization details
Contacts and Features	View contacts and features
Species (Isotype)	human
Patient	PC76
Immunogen	HIV-1 infection
Keywords	antibody binding site, antibody generation, antibody lineage, antibody sequence, mutation acquisition, neutralization, structure

Notes

Showing 2 of 2 notes.

PCDN38A: The study compared the binding characteristics of V3-glycan antibodies, specifically PGT121, PGT128, PGT135, PCDN38A, and 3 newly-derived lineages of mAbs from Donor N170. The gene usage for PCDN38A is given as: IGHV 4-34*01, IGHJ 5*01, IGLV K3-20*01, IGLJ K1*01. Longo2016 (antibody binding site, antibody sequence)
38A: For the first time, a lineage of 12 bnAbs targeting the Env high-mannose patch were isolated from donor PC76. All mAbs exhibited binding to wild type gp120 and reduced binding to N332A gp120. Neutralization breadth increased for Abs isolated from later timepoints, with 33A, 38A and 38B being the most mature. This was due to a combination of mutations arising in autologous virus, specifically E328K/A328K and deletion of glycosylation site N335, a unique feature of PC76 autologous virus, that increased viral sensitivity to NAb. The final route of escape however, was elimination of a glycan site at HIV-1 N332 or N301. Ab 38A was isolated from serum derived at 38 months post-infection. It neutralized 36% of a 110-pseudoviral panel of diverse clades; and 70.3% of a 37-virus neutralization panel at IC₅₀ geometric mean of 0.10 µg/ml MacLeod2016 (antibody generation, mutation acquisition, neutralization, structure, antibody lineage)

References

Showing 2 of 2 references.

Isolation Paper
MacLeod2016 Daniel T. MacLeod, Nancy M Choi, Bryan Briney, Fernando Garces, Lorena S. Ver, Elise Landais, Ben Murrell, Terri Wrin, William Kilembe, Chi-Hui Liang, Alejandra Ramos, Chaoran B Bian, Lalinda Wickramasinghe, Leopold Kong, Kemal Eren, Chung-Yi Wu, Chi-Huey Wong, IAVI Protocol C Investigators and The IAVI African HIV Research Network, Sergei L. Kosakovsky-Pond, Ian A. Wilson, Dennis R. Burton, and Pascal Poignard. Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch. Immunity, 44(5):1215-1226, 17 May 2016. PubMed ID: 27192579. Show all entries for this paper.

Longo2016 Nancy S. Longo, Matthew S. Sutton, Andrea R. Shiakolas, Javier Guenaga, Marissa C. Jarosinski, Ivelin S. Georgiev, Krisha McKee, Robert T. Bailer, Mark K. Louder, Sijy O'Dell, Mark Connors, Richard T. Wyatt, John R. Mascola, and Nicole A. Doria-Rose. Multiple Antibody Lineages in One Donor Target the Glycan-V3 Supersite of the HIV-1 Envelope Glycoprotein and Display a Preference for Quaternary Binding. J. Virol., 90(23):10574-10586, 1 Dec 2016. PubMed ID: 27654288. Show all entries for this paper.