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Displaying record number 3387

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MAb ID	10E8xPG9-16
HXB2 Location	Env	Env Epitope Map
Author Location	Env
Epitope
Ab Type	gp120 CD4BS, gp41 MPER (membrane proximal external region)
Neutralizing	P (tier 2)
Species (Isotype)	human
Patient
Immunogen	virus
Keywords	antibody generation, bispecific/trispecific, immunotherapy, neutralization

Notes

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10E8xPG9-16: Bispecific IgGs were produced, composed of independent antigen-binding fragments with a common Fc region. Parental antibodies of several classes were assessed (VRC07, 10E8, PGT121, PG9-16RSH). A bispecific antibody composed of VRC07 x PG9-16 displayed the most favorable profile, neutralizing 97% of viruses with a median IC₅₀ of 0.055 ug/ml. This bispecific IgG also demonstrated pharmacokinetic parameters comparable to those of the parental bNAbs when administered to rhesus macaques. These results suggest that IgG-based bispecific antibodies are promising candidates for HIV prevention and treatment. Against a panel of 206 resistant and sensitive viruses, 10E8 and PG916 neutralize with median IC₈₀s of 2.23 and 0.233 µg/ml. Bispecific, median neutralization is 0.518; while in physical combination with each other, median neutralization of the antibodies is 0.269 µg/ml. Asokan2015 (antibody generation, neutralization, immunotherapy, bispecific/trispecific)

References

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Isolation Paper
Asokan2015 M. Asokan, R. S. Rudicell, M. Louder, K. McKee, S. O'Dell, G. Stewart-Jones, K. Wang, L. Xu, X. Chen, M. Choe, G. Chuang, I. S. Georgiev, M. G. Joyce, T. Kirys, S. Ko, A. Pegu, W. Shi, J. P. Todd, Z. Yang, R. T. Bailer, S. Rao, P. D. Kwong, G. J. Nabel, and J. R. Mascola. Bispecific Antibodies Targeting Different Epitopes on the HIV-1 Envelope Exhibit Broad and Potent Neutralization. J. Virol., 89(24):12501-12512, Dec 2015. PubMed ID: 26446600. Show all entries for this paper.