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Displaying record number 3317

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MAb ID	179NC75
HXB2 Location	Env	Env Epitope Map
Author Location	Env
Epitope
Ab Type	gp120 CD4BS
Neutralizing	P (tier 2) View neutralization details
Contacts and Features	View contacts and features
Species (Isotype)	human
Patient	EB179
Immunogen	HIV-1 infection
Country	United States
Keywords	antibody binding site, antibody generation, escape, neutralization, review

Notes

Showing 2 of 2 notes.

179NC75: This review classified and mapped the binding regions of 32 bNAbs isolated 2010-2016. Wu2016 (review)
179NC75: Donor EB179 was a long-term non-progressor with high serum neutralization breadth and potency. From Clone 1 among 8 B-cell clones, 179NC75 had the highest neutralization, especially to Clade B virus, neutralizing 70% of a clade-B pseudovirus panel and 6 out of 9 cross-clade Env pseudoviruses. Binding studies revealed that 179NC75 is a glycan-dependent antibody that binds to the CD4bs in a way that involves the Asn276 residue and depends on the presence of complex glycans, in contrast to most CD4bs bNAbs which belong to the VRC01 class. It belongs to the same sub-class of CDRH3-dominated CD4bs antibodies as HJ16. Here in vitro studies in HIV-1_YU2-infected humanized mice, show that 179NC75 treatment causes a drop in viral load (VL). A rebound in VL is due to escape mutations in the virus, either a deletion on N276 glycan or a glycan shift from position 276 to 460. Freund2015 (antibody binding site, antibody generation, neutralization, escape)

References

Showing 2 of 2 references.

Isolation Paper
Freund2015 Natalia T. Freund, Joshua A. Horwitz, Lilian Nogueira, Stuart A. Sievers, Louise Scharf, Johannes F. Scheid, Anna Gazumyan, Cassie Liu, Klara Velinzon, Ariel Goldenthal, Rogier W. Sanders, John P. Moore, Pamela J. Bjorkman, Michael S. Seaman, Bruce D. Walker, Florian Klein, and Michel C. Nussenzweig. A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo. PLoS Pathog, 11(10):e1005238, Oct 2015. PubMed ID: 26516768. Show all entries for this paper.

Wu2016 Xueling Wu and Xiang-Peng Kong. Antigenic Landscape of the HIV-1 Envelope and New Immunological Concepts Defined by HIV-1 Broadly Neutralizing Antibodies. Curr. Opin. Immunol., 42:56-64, Oct 2016. PubMed ID: 27289425. Show all entries for this paper.