Search Antibody Database

Found 1 matching record:

Displaying record number 2897

Download this epitope record as JSON.

MAb ID	CH21
HXB2 Location	Env	Env Epitope Map
Author Location
Epitope
Subtype	B, CRF01_AE
Ab Type	gp120
Neutralizing	L
Species (Isotype)	human(IgG1)
Patient
Immunogen	vaccine
Country	Thailand
Keywords	antibody binding site, antibody generation, antibody polyreactivity, vaccine-induced immune responses

Notes

Showing 2 of 2 notes.

CH21: A panel of Env-specific mAbs was isolated from 6 HIV1-infected lactating women. Antibodies in colostrum may help prevent mucosal infection of the infant, so this study aimed to define milk IgGs for future vaccination strategies to reduce HIV transmission during lactation. Despite the high rate of VH 1-69 usage among colostrum Env specific B cells, it did not correlate with distinct gp120 epitope specificity or function. CH21 was compared to the newly-derived mAbs; it had no cross-reactivity with gut bacteria. Jeffries2016 (antibody polyreactivity)
CH21: NAb response was compared in 2 HIV-1 vaccine efficacy trials in which either partial protection (RV144) or no protection (Vax003) was seen. The peak NAb response in RV144 was substantially weaker than the peak response in Vax003, suggesting that either weak neutralizing antibody responses can be partially protective in low-risk heterosexual populations or that the modest efficacy seen in RV144 was mediated by other immune responses. MAb CH21 was vaccine-induced antibody, isolated from subject T141485 in RV144 trial. CH21 used V-gene segment, had no detectable neutralizing activity against tier 2 viruses, but neutralized subtype B tier 1 viruses, and did not bind to any linear epitopes spanning gp120, indicating a conformational epitope. Montefiori2012 (antibody binding site, antibody generation, vaccine-induced immune responses)

References

Showing 2 of 2 references.

Isolation Paper
Montefiori2012 David C. Montefiori, Chitraporn Karnasuta, Ying Huang, Hasan Ahmed, Peter Gilbert, Mark S. de Souza, Robert McLinden, Sodsai Tovanabutra, Agnes Laurence-Chenine, Eric Sanders-Buell, M. Anthony Moody, Mattia Bonsignori, Christina Ochsenbauer, John Kappes, Haili Tang, Kelli Greene, Hongmei Gao, Celia C. LaBranche, Charla Andrews, Victoria R. Polonis, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Jaranit Kaewkungwal, Steve G. Self, Phillip W. Berman, Donald Francis, Faruk Sinangil, Carter Lee, Jim Tartaglia, Merlin L. Robb, Barton F. Haynes, Nelson L. Michael, and Jerome H. Kim. Magnitude and Breadth of the Neutralizing Antibody Response in the RV144 and Vax003 HIV-1 Vaccine Efficacy Trials. J. Infect. Dis., 206(3):431-441, 1 Aug 2012. PubMed ID: 22634875. Show all entries for this paper.

Jeffries2016 T. L. Jeffries, Jr., C. R. Sacha, J. Pollara, J. Himes, F. H. Jaeger, S. M. Dennison, E. McGuire, E. Kunz, J. A. Eudailey, A. M. Trama, C. LaBranche, G. G. Fouda, K. Wiehe, D. C. Montefiori, B. F. Haynes, H.-X. Liao, G. Ferrari, S. M. Alam, M. A. Moody, and S. R. Permar. The Function and Affinity Maturation of HIV-1 gp120-Specific Monoclonal Antibodies Derived from Colostral B Cells. Mucosal. Immunol., 9(2):414-427, Mar 2016. PubMed ID: 26242599. Show all entries for this paper.