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MAb ID	GE148
HXB2 Location	Env	Env Epitope Map
Author Location	Env( YU2)
Epitope
Subtype	B
Ab Type	gp120 CD4BS
Neutralizing	P
Species (Isotype)	macaque(IgG)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation, antibody lineage, antibody sequence, structure, vaccine antigen design, vaccine-induced immune responses

Vaccine Details

Vaccine type	gp140F (fused form, no gp120–gp41 cleavage site)
Vaccine strain	B clade YU2
Vaccine component	Env
Adjuvant	Abisco-100, CpG oligodeoxynucleotides

Notes

Showing 3 of 3 notes.

GE148: Compared to patient-derived mAbs, vaccine-elicited mAbs are often less able to neutralize the virus, due to a less-effective angle of approach to the Env spike. This study engineered an immunogen consisting of the gp120 core in complex with a CD4bs mAb, 17b. Rabbits immunized with this antigen displayed earlier affinity maturation and better virus neutralization compared to those immunized with the gp120 core alone. The 17b antibody was shown to have a steric clash with two other CD4bs Abs, GE136 and GE148, but not with VRC01. Chen2016b (antibody binding site, vaccine antigen design, vaccine-induced immune responses, structure)
GE148: Seven CD4bs-binding MAbs from macaque F128 (GE121, GE136, GE137, GE140, GE143, GE147, and GE148) were selected for NGS sequencing to trace the longitudinal antibody lineage development in response to an HIV-1 trimer vaccine. Dai2015 (antibody sequence, antibody lineage)
GE148: This study reports the isolation of a panel of Env vaccine elicited CD4bs-directed macaque mAbs and genetic and functional features that distinguish these Abs from CD4bs MAbs produced during chronic HIV-1 infection. GE148, a CD4 specific non human primates mAb, showed high affinity to Env ligands but displayed no detectable binding to gp120-D368R. A detailed description of the immunization experiment was reported in Sundling2010. It consisted of purified gp140F trimers in adjuvant (Abisco-100+ CPG) inoculated 5 times at monthly intervals. Sundling2010,Sundling2012 (antibody generation, vaccine-induced immune responses)

References

Showing 4 of 4 references.

Isolation Paper
Sundling2010 Christopher Sundling, Mattias N. E. Forsell, Sijy O'Dell, Yu Feng, Bimal Chakrabarti, Srinivas S. Rao, Karin Loré, John R. Mascola, Richard T. Wyatt, Iyadh Douagi, and Gunilla B. Karlsson Hedestam. Soluble HIV-1 Env Trimers in Adjuvant Elicit Potent and Diverse Functional B Cell Responses in Primates. J. Exp. Med., 207(9):2003-2017, 30 Aug 2010. PubMed ID: 20679401. Show all entries for this paper.

Sundling2012 Christopher Sundling, Yuxing Li, Nick Huynh, Christian Poulsen, Richard Wilson, Sijy O'Dell, Yu Feng, John R. Mascola, Richard T. Wyatt, and Gunilla B. Karlsson Hedestam. High-Resolution Definition of Vaccine-Elicited B Cell Responses Against the HIV Primary Receptor Binding Site. Sci. Transl. Med., 4(142):142ra96, 11 Jul 2012. PubMed ID: 22786681. Show all entries for this paper.

Dai2015 Kaifan Dai, Linling He, Salar N. Khan, Sijy O'Dell, Krisha McKee, Karen Tran, Yuxing Li, Christopher Sundling, Charles D. Morris, John R. Mascola, Gunilla B. Karlsson Hedestam, Richard T. Wyatt, and Jiang Zhu. Rhesus Macaque B-Cell Responses to an HIV-1 Trimer Vaccine Revealed by Unbiased Longitudinal Repertoire Analysis. MBio, 6(6):e01375-15, 3 Nov 2015. PubMed ID: 26530382. Show all entries for this paper.

Chen2016b Yajing Chen, Richard Wilson, Sijy O'Dell, Javier Guenaga, Yu Feng, Karen Tran, Chi-I Chiang, Heather E. Arendt, Joanne DeStefano, John R. Mascola, Richard T. Wyatt, and Yuxing Li. An HIV-1 Env-Antibody Complex Focuses Antibody Responses to Conserved Neutralizing Epitopes. J. Immunol., 197(10):3982-3998, 15 Nov 2016. PubMed ID: 27815444. Show all entries for this paper.