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Displaying record number 2839

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MAb ID	33B2
HXB2 Location	gp160(304-312) DNA(7134..7160)	gp160 Epitope Map
Author Location
Epitope	RKSIRIG	Epitope Alignment
Ab Type	gp120 V3 mimotope
Neutralizing	P
Species (Isotype)	macaque(IgG1)
Patient
Immunogen	SHIV infection
Country	United States
Keywords	antibody generation, antibody sequence, binding affinity, mimotopes, neutralization

Notes

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• 33B2: mAbs with predetermined specificity were isolated from rhesus monkeys (RM) using differential biopanning method. Fluorescent mimotopes resembling V3 loop were used as baits to isolate single memory B cells. Among the 11 mAbs, 33B2 neutralized 75% HIV tier1 strains, but only 7% of tier2 strains, similar to neutralizing profiles of other anti-V3 Abs. The frequency of amino acid mutations in VH and VL domains of 33B2 was lower than anti-CD4 mAbs, but comparable to anti-V3 mAbs and b12. Sholukh2012 (antibody generation, mimotopes, neutralization, binding affinity, antibody sequence)

References

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Isolation Paper
Sholukh2012 Anton M. Sholukh, Muhammad M. Mukhtar, Michael Humbert, Sosthène S. Essono, Jennifer D. Watkins, Hemant K. Vyas, Vivekanandan Shanmuganathan, Girish Hemashettar, Maria Kahn, Shiu-Lok Hu, David C. Montefiori, Victoria R. Polonis, Peter H. Schur, and Ruth M. Ruprecht. Isolation of Monoclonal Antibodies with Predetermined Conformational Epitope Specificity. PLoS One, 7(6):e38943, 2012. PubMed ID: 22737224. Show all entries for this paper.