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MAb ID	CAP88-CH06
HXB2 Location	Env	Env Epitope Map
Author Location
Epitope
Subtype	C
Ab Type	gp120 C3
Neutralizing	P
Species (Isotype)	(IgA1)
Patient	CAP88
Immunogen	HIV-1 infection
Country	South Africa
Keywords	antibody generation, autologous responses, binding affinity, escape

Notes

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CAP88-CH06: Autologous neutralizing MAb CAP-CH06 was isolated from individual CAP88 who developed a potent neutralizing-antibody response within 3 months of infection, using a differential antigen-specific memory B-cell sorting strategy with a chimeric gp120 where the C3 region of the autologous virus was grafted into an unrelated gp120. CAP88-CH06 is an IgA1 antibody but was cloned as an IgG1 antibody. CAP88-CH06 neutralized the CAP88 transmitted/founder virus and viruses from acute infection but was unable to neutralize CAP88 viruses isolated at 6 and 12 months postinfection, although escape did not result in loss of binding. The escape was mediated by 2 amino acid changes in the alpha-2 helix of C3: introduction of an N-linked glycan at position 339 that occluded the epitope, and a charge change (either E343K or E350K). Gray2011 (antibody generation, autologous responses, escape, binding affinity)

References

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Isolation Paper
Gray2011 Elin S. Gray, M. Anthony Moody, Constantinos Kurt Wibmer, Xi Chen, Dawn Marshall, Joshua Amos, Penny L. Moore, Andrew Foulger, Jae-Sung Yu, Bronwen Lambson, Salim Abdool Karim, John Whitesides, Georgia D. Tomaras, Barton F. Haynes, Lynn Morris, and Hua-Xin Liao. Isolation of a Monoclonal Antibody That Targets the alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual. J. Virol., 85(15):7719-7729, Aug 2011. PubMed ID: 21613396. Show all entries for this paper.