Search Antibody Database

Found 1 matching record:

Displaying record number 2536

Download this epitope record as JSON.

MAb ID	2D3 (419 2D3)
HXB2 Location	gp160(419-439) DNA(7479..7541)	gp160 Epitope Map
Author Location
Epitope	`RIKQIINMWQEVGKAMYAPPI`	Epitope Alignment
Epitope Name	419
Ab Type	gp120 adjacent to CD4BS
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords	antibody sequence, binding affinity, mimotopes, structure

Vaccine Details

Vaccine type	peptide
Vaccine strain	B clade HXB2
Vaccine component	CD4BS, Env

Notes

Showing 2 of 2 notes.

419: VH5-51 gene segment was used by 18 of 51 (35%) anti-V3 MAbs. This study analyzed the crystal structure of 5 Fabs encoded by VH5-51/VL lambda genes. Each Fab interacted with key residues at the same 7 positions in the crown of the V3 loop, although the amino acids could vary, suggesting that while V3 is variable in sequence and structurally flexible, a common structure is retained across strains. All 18 VH5-51 using MAbs were studied with a constrained peptide mimotope which preserved the 3D of the VH5-51 derived MAbs 2219, 2557, 1006, but did not react with other anti-V3 MAbs that recognize different V3 epitopes. 14/18 (this MAb included) were reactive with the mimotope, compared to only 1/30 non-VH5-51 MAbs. Gorny2011 (mimotopes, antibody sequence, structure)
2D3: A strategy is described for eliciting antibodies in mice against selected cryptic, conformationally dependent conserved epitopes of gp120 by immunizing with multiple identical copies of covalently linked multiple copy peptides (MCPs) representing 3 different domains of gp120. 419 MCP MAb 2D3, and 426 MCP MAb 11A8 cross competed for binding to gp120 and they only bound to native but not denatured gp120. 2D3 bind to gp120s from other clades with lower affinities compared to clade B reflecting differences in the primary sequences. Kelker2010 (binding affinity)

References

Showing 2 of 2 references.

Kelker2010 Hanna C. Kelker, Vincenza R. Itri, and Fred T. Valentine. A Strategy for Eliciting Antibodies against Cryptic, Conserved, Conformationally Dependent Epitopes of HIV Envelope Glycoprotein. PLoS One, 5(1):e8555, 2010. PubMed ID: 20052405. Show all entries for this paper.

Gorny2011 Miroslaw K. Gorny, Jared Sampson, Huiguang Li, Xunqing Jiang, Maxim Totrov, Xiao-Hong Wang, Constance Williams, Timothy O'Neal, Barbara Volsky, Liuzhe Li, Timothy Cardozo, Phillipe Nyambi, Susan Zolla-Pazner, and Xiang-Peng Kong. Human Anti-V3 HIV-1 Monoclonal Antibodies Encoded by the VH5-51/VL Lambda Genes Define a Conserved Antigenic Structure. PLoS One, 6(12):e27780, 2011. PubMed ID: 22164215. Show all entries for this paper.