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Displaying record number 2534

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MAb ID	11A8 (426 11A8)
HXB2 Location	gp160(426-441) DNA(7500..7547)	gp160 Epitope Map
Author Location
Epitope	MWQEVGKAMYAPPIEG	Epitope Alignment
Epitope Name	426
Ab Type	gp120 adjacent to CD4BS
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords	binding affinity, cryptic epitope, neutralization

Vaccine Details

Notes

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• 11A8: A strategy is described for eliciting antibodies in mice against selected cryptic, conformationally dependent conserved epitopes of gp120 by immunizing with multiple identical copies of covalently linked multiple copy peptides (MCPs) representing 3 different domains of gp120. 419 MCP MAb 2D3, and 426 MCP MAb 11A8 cross competed for binding to gp120 and they only bound to native but not denatured gp120. 11A8 bound to gp120s from other clades with lower affinities compared to clade B reflecting differences in the primary sequences. Preincubation of gp120 with high concentrations of MAb 11A8 to the 426-441 epitope resulted in a small inhibition of CD4 binding to gp120 that suggested that the 11A8 epitope is cryptic in gp120. Control experiments demonstrated that the binding of 11A8 to infected cells and neutralization of SF162 by 11A8 is specifically blocked by preincubation of antibody with homologous MCP. Kelker2010 (neutralization, binding affinity, cryptic epitope)

References

Showing 1 of 1 reference.

Kelker2010 Hanna C. Kelker, Vincenza R. Itri, and Fred T. Valentine. A Strategy for Eliciting Antibodies against Cryptic, Conserved, Conformationally Dependent Epitopes of HIV Envelope Glycoprotein. PLoS One, 5(1):e8555, 2010. PubMed ID: 20052405. Show all entries for this paper.