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Displaying record number 2532

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MAb ID	Fab SJS-R1 (scFv SJS-R1)
HXB2 Location	Rev DNA(5970..6045,8379..8479)	Rev Epitope Map
Author Location	Rev(1-59)
Epitope
Ab Type	N-term
Neutralizing
Species (Isotype)	human, rabbit
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation, binding affinity, structure

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade BH10
Vaccine component	Rev
Adjuvant	Ribi adjuvant (MPL+TDM) (RIBI)

Notes

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Fab SJS-R1: The preparation, characterization, and crystallization of Fab SJS-R1 and the corresponding high-affinity single-chain antibody fragment (scFv) are described. This is the first time that a Fab with rabbit variable domains and human constant domains derived by phage display has been produced against an HIV-1 protein. The antibody binds with very high affinity to a unique conformational N-term Rev epitope. Both the Fab and its scFv derivative potently depolymerize Rev filaments indicating that their binding blocks a protein-protein interaction interface. The Fab/Rev complex is stable and readily forms crystals suitable for structural determination. Stahl2010 (antibody binding site, antibody generation, binding affinity, structure)

References

Showing 1 of 1 reference.

Isolation Paper
Stahl2010 Stephen J. Stahl, Norman R. Watts, Christoph Rader, Michael A. DiMattia, Rose G. Mage, Ira Palmer, Joshua D. Kaufman, Jonathan M. Grimes, David I. Stuart, Alasdair C. Steven, and Paul T. Wingfield. Generation and Characterization of a Chimeric Rabbit/Human Fab for Co-Crystallization of HIV-1 Rev. J. Mol. Biol., 397(3):697-708, 2 Apr 2010. PubMed ID: 20138059. Show all entries for this paper.