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Displaying record number 2430

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MAb ID	HGZ1
HXB2 Location	gp160	gp160 Epitope Map
Author Location	gp120
Epitope
Subtype	C
Ab Type	gp120 CD4BS
Neutralizing
Species (Isotype)	human
Patient	K500b
Immunogen	HIV-1 infection
Keywords	antibody generation, binding affinity, neutralization, optimal epitope, subtype comparisons, variant cross-reactivity

Notes

Showing 2 of 2 notes.

HGZ1: This Ab was sensitive to mutations D368A/E370A and is likely to be a conventional anti-CD4bs Ab. Pietzsch2010a (optimal epitope, variant cross-reactivity, binding affinity)
HGZ1: This MAb was isolated from memory B cells of HIV-1 infected donor using an improved EBV immortalization method combined with a broad screening strategy. HGZ1 bound to Env proteins from clades A, B, BC, C and F. It cross-competed with mAbs HJ13 and b12 for binding to gp120. HGZ1 neutralized 8/20 isolates in the HOS-based assay, 3 Tier 1 and 5 Tier 2 isolates belonging to clades A, B, C and AG. In TZM-bl assay, HGZ1 neutralized 10/46 isolates, 6 Tier 1 and 4 Tier 2 isolates belonging to clades B, C and AG. Corti2010 (antibody generation, neutralization, variant cross-reactivity, binding affinity, subtype comparisons)

References

Showing 2 of 2 references.

Isolation Paper
Corti2010 Davide Corti, Johannes P. M. Langedijk, Andreas Hinz, Michael S. Seaman, Fabrizia Vanzetta, Blanca M. Fernandez-Rodriguez, Chiara Silacci, Debora Pinna, David Jarrossay, Sunita Balla-Jhagjhoorsingh, Betty Willems, Maria J. Zekveld, Hanna Dreja, Eithne O'Sullivan, Corinna Pade, Chloe Orkin, Simon A. Jeffs, David C. Montefiori, David Davis, Winfried Weissenhorn, Áine McKnight, Jonathan L. Heeney, Federica Sallusto, Quentin J. Sattentau, Robin A. Weiss, and Antonio Lanzavecchia. Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals. PLoS One, 5(1):e8805, 2010. PubMed ID: 20098712. Show all entries for this paper.

Pietzsch2010a John Pietzsch, Johannes F. Scheid, Hugo Mouquet, Florian Klein, Michael S. Seaman, Mila Jankovic, Davide Corti, Antonio Lanzavecchia, and Michel C. Nussenzweig. Human Anti-HIV-Neutralizing Antibodies Frequently Target a Conserved Epitope Essential for Viral Fitness. J. Exp. Med., 207(9):1995-2002, 30 Aug 2010. PubMed ID: 20679402. Show all entries for this paper.