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Displaying record number 2075

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MAb ID	3697
HXB2 Location	Env	Env Epitope Map
Author Location	gp120 (V3)
Epitope
Subtype	G
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing
Species (Isotype)	human(IgG1λ)
Patient
Immunogen	HIV-1 infection
Country	Cameroon
Keywords	antibody generation, antibody sequence, binding affinity, structure, vaccine antigen design

Notes

Showing 2 of 2 notes.

3697: Two V3-scaffold immunogen constructs were designed and expressed using 3D structures of cholera toxin B (CTB), V3 in the gp120 context, and V3 bound to 447-52D MAb. The construct (V3-CTB) presenting the complete V3 was recognized by 3697 MAb and by the large majority of other MAbs (18/24), indicating correctly folded and exposed MAb epitopes. V3-CTB induced V3-binding Abs and Abs displaying cross-clade neutralizing activity in immunized rabbits. Short V3-CTB construct, presenting a V3 fragment in conformation observed in complex with 447-52D, was not recognized by 3697. Totrov2010 (vaccine antigen design, binding affinity, structure)
3697: This Ab was derived from one of 12 individuals infected with non-B subtypes from Cameroon and India. The Ig usage for variable heavy chain of this Ab was as follows: IGHV:1-f*01, IGHD:5-24, D-RF:3, IGHJ:4. There was a preferential usage of the VH5-51 gene segment for V3 Abs. The usage of the VH4 family for the V3 Abs was restricted to only one gene segment, VH4-59, and the VH3 gene family was used at a significantly lower level by these Abs. The V3 Abs preferentially used the JH3 and D2-15 gene segments. Gorny2009 (antibody generation, antibody sequence)

References

Showing 2 of 2 references.

Isolation Paper
Gorny2009 Miroslaw K. Gorny, Xiao-Hong Wang, Constance Williams, Barbara Volsky, Kathy Revesz, Bradley Witover, Sherri Burda, Mateusz Urbanski, Phillipe Nyambi, Chavdar Krachmarov, Abraham Pinter, Susan Zolla-Pazner, and Arthur Nadas. Preferential Use of the VH5-51 Gene Segment by the Human Immune Response to Code for Antibodies against the V3 Domain of HIV-1. Mol. Immunol., 46(5):917-926, Feb 2009. PubMed ID: 18952295. Show all entries for this paper.

Totrov2010 Maxim Totrov, Xunqing Jiang, Xiang-Peng Kong, Sandra Cohen, Chavdar Krachmarov, Aidy Salomon, Constance Williams, Michael S. Seaman, Ruben Abagyan, Timothy Cardozo, Miroslaw K. Gorny, Shixia Wang, Shan Lu, Abraham Pinter, and Susan Zolla-Pazner. Structure-Guided Design and Immunological Characterization of Immunogens Presenting the HIV-1 gp120 V3 Loop on a CTB Scaffold. Virology, 405(2):513-523, 30 Sep 2010. PubMed ID: 20663531. Show all entries for this paper.