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Displaying record number 195

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MAb ID	5F8
HXB2 Location	Pol(716-731) DNA(4230..4277)	Pol Epitope Map
Author Location	Integrase(1-16 HXB2)
Epitope	FLDGIDKAQDEHEKYH	Epitope Alignment
Subtype	B
Ab Type	N-term
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Notes

Showing 2 of 2 notes.

• 5F8: One of a large set of MAbs that interact with the N-terminal part of integrase: 1C4, 2C11, 2E3, 3E11, 3F9, 5F8, 6G5, 7B6, 7C6 -- these MAbs inhibit end processing and DNA joining, but had little effect on integration activities. Nilsen1996
• 5F8: MAb interferes with integrase binding to DNA. Haugan1995

References

Showing 2 of 2 references.

Haugan1995 I. R. Haugan, B. M. Nilsen, S. Worland, L. Olsen, and D. E. Helland. Characterization of the DNA-Binding Activity of HIV-1 Integrase Using a Filter Binding Assay. Biochem. Biophys. Res. Commun., 217:802-810, 1995. PubMed ID: 8554601. Show all entries for this paper.

Nilsen1996 B. M. Nilsen, I. R. Haugan, K. Berg, L. Olsen, P. O. Brown, and D. E. Helland. Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Integrase: Epitope Mapping and Differential Effects of Integrase Activities In Vitro. J. Virol., 70:1580-1587, 1996. In this study, 17 anti-integrase murine Mabs were generated and epitopes were mapped by deletion mutations and peptide scanning. The ability of MAb binding to inhibit (or stimulate) end-processing, DNA joining, reintegration, and disintegration enzyme functions it in vitro was determined. PubMed ID: 8627677. Show all entries for this paper.