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Displaying record number 1856

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MAb ID	p5F1
HXB2 Location	Gag(329-350) DNA(1774..1839)	Gag Epitope Map
Author Location	Gag(329-350)
Research Contact	Dr. Yongtang Zheng, Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Kunming, Yunnan, China
Epitope	`DCKTILKALGPAATLEEMMTAC`	Epitope Alignment
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG1)
Patient
Immunogen	vaccine
Keywords	antibody generation, assay or method development, binding affinity

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade
Vaccine component	p24 Gag
Adjuvant	Complete Freund's Adjuvant (CFA), Incomplete Freund's Adjuvant (IFA)

Notes

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p5F1: MAb p5F1 was derived from hybridoma from mice immunized with HIV-1 p24. p5F1 reacted with p24 from HIV-1 IIIB, Ada-M, 74V, and KM018 strains. p5F1 recognized peptide DC-22, Gag 329-350. Detection limit for rp24 in a modified sandwich ELISA of p5F1 was about 15 ng/ml, while p5F1 could detect as low as 40 pg/ml of p24 in standard ELISA. p5F1 showed good specificity and high sensitivity in a modified sandwich ELISA with rabbit anti-p24 serum, indicating its potential use for measurement of p24 antigen levels in research. Liu2007 (antibody generation, assay or method development, binding affinity)

References

Showing 1 of 1 reference.

Isolation Paper
Liu2007 Guangjie Liu, Jianping Wang, Jianchun Xiao, Zhiwei Zhao, and Yongtang Zheng. Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein. Cell. Mol. Immunol., 4(3):203-8, Jun 2007. PubMed ID: 17601374. Show all entries for this paper.