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Displaying record number 171

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MAb ID	7C4
HXB2 Location	Pol	Pol Epitope Map
Author Location	RT(252-335)
Epitope
Epitope Name	HRSINE
Ab Type	RT thumb domain
Neutralizing
Species (Isotype)	mouse(IgG2a)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody generation

Vaccine Details

Vaccine type	vaccinia
Vaccine strain	B clade HXB2
Vaccine component	RT

Notes

Showing 3 of 3 notes.

7C4: Fabs 5F and 5G both recognize the same immunodominant neutralizing RT epitope in the region of the template primer-binding site in the thumb domain recognized by MAb 7C4 -- sequencing revealed the heavy chains and light chains of 7C4, 5G and 7C4 are related. Ohba2001
7C4: 7C4 inhibits RT from HIV-1 strains IIIB, Bru, and IMS-1 but not HIV-2 strains GH-1 and LAV-2, SIV MAC, nor SIV MND. Chiba1997
7C4: 7C4 was produced from a hybridoma cell line derived from a BALB/c mouse repeatedly immunized with RT in a vaccinia construct, and was found to inhibit RT through binding to the template primer-binding site, a possible target for RT inhibitors. Chiba1996 (antibody binding site, antibody generation)

References

Showing 3 of 3 references.

Isolation Paper
Chiba1996 J. Chiba, A. Yamaguchi, Y. Suzuki, M. Nakano, W. Zhu, H. Ohba, A. Saito, H. Shinagawa, Y. Yamakawa, T. Kobayashi, and T. Kurata. A novel neutralization epitope on the `thumb' subdomain of human immunodeficiency virus type 1 reverse transcriptase revealed by a monoclonal antibody. J. Gen. Virol., 77(12):2921--9, Dec 1996. PubMed ID: 9000082. Show all entries for this paper.

Chiba1997 J. Chiba, M. Nakano, Y. Suzuki, K. Aoyama, H. Ohba, T. Kobayashi, A. Yasuda, A. Kojima, and T. Kurata. Generation of neutralizing antibody to the reverse transcriptase of human immunodeficiency virus type 1 by immunizing of mice with an infectious vaccinia virus recombinant. J. Immunol. Methods, 207:53-60, 1997. PubMed ID: 9328586. Show all entries for this paper.

Ohba2001 H. Ohba, T. Soga, T. Tomozawa, Y. Nishikawa, A. Yasuda, A. Kojima, T. Kurata, and J. Chiba. An immunodominant neutralization epitope on the `thumb' subdomain of human immunodeficiency virus type 1 reverse transcriptase revealed by phage display antibodies. J. Gen. Virol., 82(Pt 4):813--20, Apr 2001. URL: http://vir.sgmjournals.org/cgi/content/full/82/4/813. PubMed ID: 11257186. Show all entries for this paper.