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Displaying record number 1596

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MAb ID	μ5.5 (5.5, mu5.5, Rμ5.5)
HXB2 Location	gp160(309-319) DNA(7149..7181)	gp160 Epitope Map
Author Location	gp120( MN)
Epitope	IHIGPGRAFYT	Epitope Alignment
Ab Type	gp120 V3 // V3 glycan (V3g)
Neutralizing	L P
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen
Keywords	antibody binding site, binding affinity, neutralization, variant cross-reactivity

Notes

Showing 5 of 5 notes.

• Rμ5.5: Postinfection passive immunization of SHIV-infected monkeys with KD-247 resulted in suppressed viral loads and protection against loss of CD4+ T cells. One of six monkeys given KD-247 could not maintain Ab concentrations, and binding of KD-247 to antigen peptides decreased by 60% after reaction with the plasma from this monkey, suggesting presence of anti-idiotype Abs. The Abs from this plasma did not react with Rμ5.5. Murakami2009
• Rμ5.5: This MAb was used as a positive control in KD-247 studies. HIV-1 plasma and PBMC clone sequences from two patients were used for prediction of the effect of KD-247 against patient's primary isolates. In patient 1 all clones matched epitope sequence recognized by KD-247 while 5 out of 7 plasma, and 2 out of 22 PBMC sequences matched the sequence recognized by Rμ5.5. In the second patient only a small portion of the quasi-species was recognized by Rμ5.5. Matsushita2005 (antibody binding site)
• μ5.5: This Ab was shown not to neutralize HIV-1 MNp in spite of the fact that HIV-1 MNp V3 tip sequence is identical to the V3 sequence of this Ab's epitope, suggesting that the neutralization epitope of HIV-1 MNp may be narrower than that of the V3 epitope of Rμ5.5. Rμ5.5 also did not neutralize HIV-1 AD8, SHIV 89.6 and SHIV C2/1. The affinity of this Ab was tested for HIV-1 MN. Eda2006a (neutralization, variant cross-reactivity, binding affinity)
• μ5.5: Rμ5.5 is a humanized antibody of mouse MAb m5.5 -- neutralized primary isolates with similar V3 loops -- passive transfer of MAb to SCID-hu or hu-PBL-SCID mice conferred protection. Okamoto1998
• mu5.5: sCD4 causes loss of IIIB type-specificity for MAb 0.5beta, allowing binding and neutralization of MN, in contrast to MAb μ5.5. Maeda1992

References

Showing 5 of 5 references.

Eda2006a Yasuyuki Eda, Mari Takizawa, Toshio Murakami, Hiroaki Maeda, Kazuhiko Kimachi, Hiroshi Yonemura, Satoshi Koyanagi, Kouichi Shiosaki, Hirofumi Higuchi, Keiichi Makizumi, Toshihiro Nakashima, Kiyoshi Osatomi, Sachio Tokiyoshi, Shuzo Matsushita, Naoki Yamamoto, and Mitsuo Honda. Sequential Immunization with V3 Peptides from Primary Human Immunodeficiency Virus Type 1 Produces Cross-Neutralizing Antibodies against Primary Isolates with a Matching Narrow-Neutralization Sequence Motif. J. Virol., 80(11):5552-5562, Jun 2006. PubMed ID: 16699036. Show all entries for this paper.

Maeda1992 Y. Maeda, S. Matsushita, T. Hattori, T. Murakami, and K. Takatsuki. Changes in the Reactivity and Neutralizing Activity of a Type-Specific Neutralizing Monoclonal Antibody Induced by Interaction of Soluble CD4 with gp120. AIDS Res. Hum. Retroviruses, 8:2049-2054, 1992. PubMed ID: 1493053. Show all entries for this paper.

Matsushita2005 Shuzo Matsushita, Soichiro Takahama, Junji Shibata, Tetsuya Kimura, Koichi Shiozaki, Yasuyuki Eda, Atsushi Koito, Toshio Murakami, and Kazuhisa Yoshimura. Ex Vivo Neutralization of HIV-1 Quasi-Species by a Broadly Reactive Humanized Monoclonal Antibody KD-247. Hum. Antibodies, 14(3-4):81-88, 2005. PubMed ID: 16720978. Show all entries for this paper.

Murakami2009 Toshio Murakami, Yasuyuki Eda, Tadashi Nakasone, Yasushi Ami, Kenji Someya, Naoto Yoshino, Masahiko Kaizu, Yasuyuki Izumi, Hajime Matsui, Katsuaki Shinohara, Naoki Yamamoto, and Mitsuo Honda. Postinfection Passive Transfer of KD-247 Protects against Simian/Human Immunodeficiency Virus-Induced CD4+ T-Cell Loss in Macaque Lymphoid Tissue. AIDS, 23(12):1485-1494, 31 Jul 2009. PubMed ID: 19528788. Show all entries for this paper.

Okamoto1998 Y. Okamoto, Y. Eda, A. Ogura, S. Shibata, T. Amagai, Y. Katsura, T. Asano, K. Kimachi, K. Makizumi, and M. Honda. In SCID-hu Mice, Passive Transfer of a Humanized Antibody Prevents Infection and Atrophic Change of Medulla in Human Thymic Implant Due to Intravenous Inoculation of Primary HIV-1 Isolate. J. Immunol., 160:69-76, 1998. PubMed ID: 9551957. Show all entries for this paper.