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Displaying record number 1543

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MAb ID	polyclonal
HXB2 Location	Env	Env Epitope Map
Author Location	Env( Consensus B)
Research Contact	Beatrice Hahn, University of Alabama, Birmingham, bhahn@uab.edu
Epitope
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	guinea pig
Patient
Immunogen	vaccine
Keywords	antibody binding site, co-receptor, neutralization, vaccine antigen design, variant cross-reactivity

Vaccine Details

Vaccine type	DNA
Vaccine strain	B clade consensus, B clade CAAN5342, B clade WITO4160
Vaccine component	Env

Notes

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Four consensus B Env constructs: full length gp160, uncleaved gp160, truncated gp145, and N-linked glycosylation-site deleted (gp160-201N/S) were compared. All were packaged into virions, and all but the fusion defective uncleaved version mediated infection using the CCR5 co-receptor. DNA vaccinations of the four constructs were compared to two wildtype B clade isolates, CAAN5342 and WITO4160. The binding antibody titers elicited by the consensus proteins were 20 to 40 fold higher than to the two wildtype strains. ConB gp145 emerged as the immunogen with the greatest breadth and magnitude of responses, WITO4160 the worst. Con B gp145 and gp160 elicted significantly more potent antibodies than the wild type vaccines against a panel of easier to neutralize tier 1 viruses, but limited neutralization of tier 2 viruses was observed with any of the immunogens. Kothe2007 (antibody binding site, co-receptor, neutralization, vaccine antigen design, variant cross-reactivity)

References

Showing 1 of 1 reference.

Kothe2007 Denise L. Kothe, Julie M Decker, Yingying Li, Zhiping Weng, Frederic Bibollet-Ruche, Kenneth P. Zammit, Maria G. Salazar, Yalu Chen, Jesus F. Salazar-Gonzalez, Zina Moldoveanu, Jiri Mestecky, Feng Gao, Barton F. Haynes, George M. Shaw, Mark Muldoon, Bette T. M. Korber, and Beatrice H. Hahn. Antigenicity and Immunogenicity of HIV-1 Consensus Subtype B Envelope Glycoproteins. Virology, 360(1):218-234, 30 Mar 2007. PubMed ID: 17097711. Show all entries for this paper.