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Displaying record number 1526

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MAb ID	1014
HXB2 Location	Env	Env Epitope Map
Author Location	gp41
Research Contact	G. Marius Clore or Carole Bewley, NIH, Bethesda, Maryland. marius@intra.niddk.nih.gov or caroleb@mail.nih.gov
Epitope
Subtype	B
Ab Type	gp41 six-helix bundle
Neutralizing
Species (Isotype)	human
Patient
Immunogen	in vitro stimulation or selection
Keywords	antibody binding site, antibody generation

Notes

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1014: This is one of nine anti-gp41 bivalent Fabs selected from a non-immune human phage display library. This Fab was classified as class A, and inhibits LAV mediated Env-mediated cell fusion with an IC50 of 36 +/-1 ug/ml (the range for the 9 new Fabs was 6-61 ug/ml -- for context, 2F5 and 2G12 were about an order of magnitude more potent in this assay than the best Fabs generated here). Class A Fabs interact with the six-helix bundle of gp41. Louis2005 (antibody binding site, antibody generation)

References

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Isolation Paper
Louis2005 John M. Louis, Carole A. Bewley, Elena Gustchina, Annie Aniana, and G. Marius Clore. Characterization and HIV-1 Fusion Inhibitory Properties of Monoclonal Fabs Obtained from a Human Non-Immune Phage Library Selected against Diverse Epitopes of the Ectodomain of HIV-1 gp41. J. Mol. Biol., 353(5):945-951, 11 Nov 2005. PubMed ID: 16216270. Show all entries for this paper.