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Displaying record number 1360

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MAb ID	polyclonal
HXB2 Location	Env	Env Epitope Map
Author Location	Env (gp160)
Epitope
Subtype	multiple
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG)
Patient
Immunogen	vaccine
Keywords	adjuvant comparison, enhancing activity, Th1, Th2, vaccine antigen design, variant cross-reactivity

Vaccine Details

Vaccine type	DNA, DNA prime with protein boost
Vaccine strain	B clade LAI, A clade 92UG031, C clade 92BR025
Vaccine component	gp160
Adjuvant	GM-CSF

Notes

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Vaccination of mice with subtype B Env raised antibodies primarily against subtype B alone, while A+B+C clade Envs raised antibodies that could neutralize the autologous B, C strains, and weakly neutralize the A strain. Serum IgG responses to gp120s including all gp120 variable regions were induced in animals vaccinated with subtypes A, B and C of HIV-1 gp160 with rGM-CSF as adjuvant. Boosting with rgp160 with CpG-ODN enhanced IgG responses, shifted the Th1/Th2 to be more balanced, and these animals made both IgG and Ig2a responses and had expanded recognition of constant regions. The B clade vaccine was LAI, and the A and C clade vaccines were actually V1-V5 of the A and C strains cloned into a LAI backbone. gp41 peptides were also recognized by sera. T cell responses to the multi-clade vaccine had enhanced cross-reactive CD4 T-cell proliferative responses, but diminished gamma IFN CD8 T-cell responses. Rollman2004 (adjuvant comparison, enhancing activity, vaccine antigen design, variant cross-reactivity, Th1, Th2)

References

Showing 1 of 1 reference.

Rollman2004 E. Rollman, J. Hinkula, J. Arteaga, B. Zuber, A. Kjerrström, M. Liu, B. Wahren, and K. Ljungberg. Multi-Subtype gp160 DNA Immunization Induces Broadly Neutralizing Anti-HIV Antibodies. Gene Ther., 11(14):1146-1154, Jul 2004. PubMed ID: 15103320. Show all entries for this paper.